Get Quote for Custom Synthetic MicroRNA Agomir/Antagomir using your sequences or Pre-designed MIRacle™ MicroRNA Agomir/Antagomir
AcceGen MIRacle™ miRNA Agomir is the advanced product of miRNA mimic, MIRacle™ miRNA Antagomir is the advanced product of miRNA Inhibitor. AcceGen MIRacle™ miRNA Agomir/Antagomir are one of most innovative research tools with superior specificity and potency for elucidating the role of microRNAs.
Compared to other common miRNA mimics and inhibitors, MIRacle™ Agomirs and Antagomirs have the higher affinity for cell membranes, so the amount of needed transfection reagent could be drastically reduced in the cell transfection experiment to reduce experimental variables and improve the cost-efficiency; MIRacle™ Agomirs and Antagomirs have the higher simulating and inhibiting effect, so these products are suitable for the animals in vivo RNAi experiment by injection, inhalation, or feeding; MIRacle™ Agomirs and Antagomirs are more resistant to degradation, so the interfering effect can last at least 1 week, even up to 5-6 weeks for long-term miRNA functional studies.
AcceGen’s microRNA agomir/antagomir synthesis service has the following advantages:
| Species | Human / Mouse / Rat | Size/Quantity | 2 OD, 4 OD, 50 OD (size by request, 1 OD corresponds to 33 ug) |
| Shipping Info | Room Temperature | Storage | -20°C / -80°C |
| Quality Control | HPLC | Label | FAM, CY3, CY5, etc. (optional) |
| Component | MIRacle™ Agomir and/or Antagomir (Product Form: Dry Powder) Agomir N.C and/or Antagomir N.C (optional) |
Application | For research use only |
| Species | Human / Mouse / Rat |
| Size/Quantity | 2 OD, 4 OD, 50 OD (size by request, 1 OD corresponds to 33 ug) |
| Shipping Info | Room Temperature |
| Storage | -20°C / -80°C |
| Quality Control | HPLC |
| Label | FAM, CY3, CY5, etc. (optional) |
| Component | MIRacle™ Agomir and/or Antagomir (Product Form: Dry Powder) Agomir N.C and/or Antagomir N.C (optional) |
| Application | For research use only |
Need a quote for MIRacle™ Agomir/Antagomir to improve your research project success or a consultation to discuss your project with our MicroRNA experts? CONTACT US NOW! We have significant bulk order discounts.
MicroRNA imperfect pair with target messenger RNAs (mRNAs) of protein-coding genes and regulate the transcription or post-transcription of their expression to involve in crucial biological processes, including development, differentiation, apoptosis, and proliferation. AcceGen MIRacle™ miRNA Agomir and Antagomir can be efficiently enriched in target cells to achieve specific and stable interference, so Agomir/Antagomir transfection into cells and injection in animals are the two main research applications. MIRacle™ miRNA Agomir/Antagomir are for research use only, however, these advanced products still have practical significance for the research of clinical trials, cancer treatments, and gene therapies, since it is speculated that miRNAs regulate one-third of human genes.
Specific microRNA Agomir/Antagomir is introduced into the cells which express the corresponding microRNA, and then it simulates the effect of microRNA to simulate/inhibit the effect of microRNA. Or the specific microRNA Agomir/Antagomir can be used to combine with the double luciferase reporter system/suppress the expression of a reporter vector, to verify the regulatory relationship between the miRNA and the target gene.
Transfection efficiency is different for different cell lines or different transfection reagents. The optimal transfection conditions still need to be determined by experiments. We suggest that the concentration range of miRNA Agomir is 1-100 nM and miRNA Antagomir is 15-100nM.
| 96 well plate | 24 well plate | 12 well plate | 6 well plate | |
| Transfection reagent | 0.2-0.6 μL | 0.5-2 μL | 1-3 μL | 2-5 μL |
| MicroRNA Agomir | 3 pmol | 15 pmol | 30 pmol | 75 pmol |
| Cell density | 6,000 cells/well | 40,000 cells/well | 80,000 cells/well | 200,000 cells/well |
| Final volume per well | 0.1 mL | 0.5 mL | 1.0 mL | 2.5 mL |
| 96 well plate | 24 well plate | 12 well plate | |
| Transfection reagent | 0.2-0.5 μL | 0.5-1 μL | 0.5-2 μL |
| MicroRNA Antagomir | 3 pmol | 15 pmol | 30 pmol |
| Cell density | 6,000 cells/well | 40,000 cells/well | 80,000 cells/well |
| Final volume per well | 0.1 mL | 0.5 mL | 1.0 mL |
Through local injection or tail vein injection in animals, miRNA Agomir/Antagomir plays a role in local or systemic high expression of target miRNA. The dose of Agomir/Antagomir varies greatly with the way of administration. The dosage regimen is referred to as follows:
Recommended dosage per dose: Agomir/Antagomir 5-80μg/g body weight;
Dosage per mouse (body weight 15-20g): Agomir/Antagomir 200nmol (Generally need 3 injections).
| Intravenous administration | suitable for heart, liver, kidney, lung, tumor tissues, and other tissues with rich blood flow | 6-week-old mouse, 45-150 μg, continuous injection for 3 days, test- 24 hours after the last injection |
| Respiratory administration | suitable for the respiratory system | N/A |
| Intraperitoneal administration | suitable for internal organs of the abdominal cavity and pelvis, pancreas, spleen, kidney, ovary, etc. | N/A |
| Intracranial administration | suitable for central nervous system research | N/A |
| Local administration | suitable for sites difficult to reach with systemic administration, such as epidermis, subcutaneous (tumor), uterine cavity, etc. | Xenograft nude mouse model, tumor Intra-injection, multi-point injection of 15μg, continuous 2-4 weeks, 2 times a week, 2-4 weeks after the first injection |
| Custom Synthesis | Pre-designed Database | Technical Support |
| Order custom synthetic miRNA Agomir/Antagomir | Search in MIRacle™ miRNA Agomir & Antagomir Database | Any questions on miRNA Agomir/Antagomir, please contact us at [email protected] |
We suggest that miRNA Agomir/Antagomir negative control is used as a reference in the experiment. The concentration and transfection conditions of the negative control are the same as those of the experimental group. Agomir N.C and Antagomir N.C can be optionally added with the synthetic miRNA Agomir/Antagomir products in your order.
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