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Tumor Cell Lines

V79

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    1

  • 218
Hamster Chinese lung male. Established after spontaneous transformation of cells isolated from the lung of a normal Chinese hamster (male). The G1 phase in these cells is either absent or very short. Consequently they have been used in studies of the G1 growth period. V79 is well established in toxicology studies. Stability of karyotype and […]
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Product Code

V-79; V 79; Strain V; V79-1

Species

Cricetulus Griseus

Cat.No

ABC-TC1270

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Cell Type

Fibroblast

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Lung

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Cricetulus Griseus Lung Cell Lines

Description

V79 is a Chinese hamster lung fibroblast cell line originally isolated from the lung tissue of a young male Chinese hamster. This adherent cell line exhibits a fibroblast-like morphology and has a doubling time of approximately 16–20 hours. Karyotypic analysis reveals an aneuploid chromosome count ranging from 20–25, with a stable modal number of 22, making it a preferred model for mutagenicity and genotoxicity studies. V79 does not produce immunoglobulin but expresses Fas antigen and demonstrates sensitivity to TNF and anti-Fas antibodies. Due to its genetic stability and high susceptibility to mutations, particularly at loci like hypoxanthine-guanine phosphoribosyl transferase (HGPRT), V79 is widely used in toxicology and radiation research.
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Citation

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Application

  • V79 cells hold significant prominence in DNA repair and damage research, primarily due to their distinct attributes, including a truncated G1 phase, karyotype stability, and the absence of endogenous cytochrome P450 expression. These characteristics make them ideal for gene toxicity assays, especially when used to express exogenous P450 enzymes from diverse species. Furthermore, their suitability for the establishment of stable mutant cell lines proves invaluable in investigating DNA repair enzymes and pathways. Despite certain concerns, V79 cells have played a pivotal role in the study of DNA damage induced by x-rays, UV light, oxidizing agents, and various environmental mutagens, thereby contributing significantly to the understanding of carcinogenic mechanisms.

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