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THLE-3 cells are a cell line derived from primary normal liver cells through infection with SV40 large T antigen. These epithelial cells were isolated from the left lobe of a donor and exhibit characteristics similar to normal adult liver epithelial cells. Notably, they are non-tumorigenic when injected into athymic nude mice and have near-diploid karyotypes while lacking alpha-fetoprotein expression. THLE-3 cells are capable of metabolizing benzo[a]pyrene, N-nitrosodimethylamine, and aflatoxin B1 into their ultimate carcinogenic metabolites that adduct DNA, indicating functional cytochrome P450 pathways. To maintain these cells, when confluent, they are subcultured using 0.05% trypsin or trypsin/EDTA at a ratio of 1:2 to 1:6, and they should be fed 2 to 3 times weekly.
Why choose THLE-3 from AcceGen?
The THLE-3 cell boasts essential features such as sterility, being free from bacteria, yeast, mycoplasma, and human pathogens like HIV, HBV, and HCV. These cells are incubated and cryopreserved under optimal conditions by expert operators, ensuring their viability. Furthermore, rigorous quality control measures are applied to guarantee the reliability and consistency of the THLE-3 cell line.
| Product Code | THLE3; Transformed Human Liver Epithelial-3 |
| Species | Human |
| Cat.No | ABC-TC1338 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Liver |
| Disease | Normal |
| Biosafety Level | 2 |
| Storage | Liquid Nitrogen |
| Product Type | Human Liver Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
THLE-3 cells serve as valuable tools in pharmacotoxicological studies and investigations into the causes and development of human hepatocellular carcinoma. As immortalized human liver cells, they provide an in vitro model for these purposes. Their application lies in being a normal cell model for various assays, allowing researchers to compare responses to chemicals or biochemical molecules between THLE-3 and hepatocellular carcinoma cells, ensuring that potential effects of substances are specifically targeted towards diseased cells while leaving healthy cells unaffected.
