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Retinal Müller Cell Line

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rMC-1 Immortalised cells constitutively express GFAP (glial fibrillary acidic protein) and CRALBP (cellular retinaldehyde-binding protein) which are present in Müller cells.Müller cells are the most abundant type of glial cells found in the vertebrate retina, and perform diverse functions that support the activity of retinal neurons. This Immortalised cell line facilitates gene expression studies in […]
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Home | Immortalized Cell Lines | Retinal Müller Cell Line

The Retinal Müller Cell Line originates from primary rat retinal Müller cells, achieved by stable transformation with SV40 antigen. While retaining key features of primary cells, they express glial fibrillary acidic protein (GFAP) and cellular retinaldehyde-binding protein (CRALBP) markers, indicative of reactive gliosis and adult retinal Müller cells, albeit at slightly diminished levels. These cells exhibit a more streamlined, elongated form with less conspicuous nuclei, and they boast an accelerated growth rate, doubling approximately every 48 to 52 hours.

 

Why choose Retinal Müller Cell Line from AcceGen?

The Retinal Müller Cell Line features high viability and quality, maintained in a sterile environment by skilled operators. Rigorous quality control involves immunocytochemistry and immunoblotting to confirm the presence of GFAP and CRALBP markers, along with verifying nuclear expression of SV40-LTA. This assures the cell line’s authenticity and suitability for diverse research applications.

Species

Rat
Cat.No

ABI-TC046D
Product Category Immortalized Cell Lines
Size/Quantity

1 vial
Cell Type

Retinal Muller Cell
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Eye
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Immortalized Cell Line

Citation

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Application

  • FOR RESEARCH USE ONLY

  • The Retinal Müller Cell Line offers a breakthrough in research, addressing the limitations of primary cultures. While primary Müller cells have been pivotal in cell biology, developmental, and electrophysiological studies, their short lifespan and susceptibility to non-neural cell contamination have imposed constraints. The establishment of a permanent Müller cell line opens doors to diverse investigations, encompassing the delineation of Müller cell-specific antigens, interactions with neurons and endothelial cells, profiling secreted growth factors and cytokines, and scrutiny of gene expression regulation. These advancements promise a deeper comprehension of Müller cell functions within the mammalian retina, heralding a new era of insightful research.

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