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Primary Cells

Rat Mammary Epithelial Cells

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Rat Mammary Epithelial Cells from AcceGen are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Mammary Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. […]
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Species

Rat

Cat.No

ABC-TC4166

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Product Category Primary Cells
Size/Quantity

1 vial

Cell Type

Epithelial

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Mammary

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Rat Primary Cells

Description

Rat Mammary Epithelial Cells from AcceGen are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Mammary Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1×10^6 cells and is delivered frozen.
Rat Mammary Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Mammary Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

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Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

  • For research use only

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