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Rat Dermal Epithelial Cells

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Product Name

Rat Dermal Epithelial Cells

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Cat.No

ABC-TC4118

Species

Rat

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Rat Dermal Epithelial Cells from AcceGen are isolated from tissue of 1-day-old neonatal laboratory Sprague–Dawley Rats. Rat Dermal Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1×10^6 cells and is delivered frozen.
Rat Dermal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin and ZO-1. Rat Dermal Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with epithelial cell cultures include the assays of cell to cell adhesion and migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Disease

Normal

Source Organ

Dermal

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement

ABM-TM4118 Rat Dermal Epithelial Cells Medium Kit

Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only

Growth Conditions

37 ℃, 5% CO2

Cell Type

Epithelial

Growth Mode

Adherent

Product Type

Rat Primary Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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