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Rat Colonic Smooth Muscle Cells

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Rat Colonic Smooth Muscle Cells from AcceGen are isolated from tissue of Sprague-Dawley Rats. Rat Colonic Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from […]
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Home | Primary Cells | Animal Primary Cells | Rat Primary cells | Rat Colonic Smooth Muscle Cells
Species

Rat
Cat.No

ABC-TC4114
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Smooth Muscle Cell
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Colonic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Rat Primary Cells

Description

Rat Colonic Smooth Muscle Cells from AcceGen are isolated from tissue of Sprague-Dawley Rats. Rat Colonic Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.
Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin.
Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen.
Repeated freezing and thawing of cells is not recommended.
Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

AcceGen Frozen Cells & Cell Lines 1 vial
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Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

  • For research use only

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