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OVMANA cells are derived from a stage IV ovarian clear cell carcinoma (OCCC) patient who received prior cisplatin treatment. These Japanese-origin cells display copy number aberrations throughout the genome and mutations in OCCC-related genes (ARID1A, PIK3CA, KRAS, PTEN). They express HNF1-β, Napsin A, and cytokeratin polypeptides (CKs 6, 7, 8, 18, 19, 20, 15, and/or 16), as well as wild-type p53. OVMANA cells can be cultured at 37 °C using RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS).
Why choose OVMANA from AcceGen?
The OVMANA cell product from AcceGen is prepared under sterile conditions to ensure its safety and purity. It exhibits high viability and quality; a significant proportion of the cells remain alive and functional after thawing. The cells can maintain a good cell state with intact morphology, physiological characteristics, and functionality. Rigorous quality control measures are implemented throughout the production process to meet defined specifications and maintain consistency. The entire operation is conducted by skilled professionals who possess expertise in cell culture techniques, ensuring the utmost care and precision. These features collectively contribute to a reliable, high-quality OVMANA cell product that meets the stringent standards of quality control and professional operation.
| Species | Human |
| Cat.No | ABC-TC0883 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Ovary |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Ovarian Cancer Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
OVMANA cells, derived from ovarian clear cell carcinoma (OCCC), have significant applications in the scientific field. They promote understanding of this rare subtype of ovarian cancer, which lacks efficient treatment options, by unraveling its molecular mechanisms and genetic abnormalities. OVMANA cells serve as a model for testing chemicals and potential drugs, evaluating their ability to induce cell death or autophagy in OCCC. This enables the identification of promising candidates for OCCC treatment. Additionally, these cells aid in investigating the high resistance of OCCC to standard chemotherapy, facilitating the development of novel therapeutics to overcome this resistance and improve patient outcomes. In summary, OVMANA cells contribute to understanding OCCC, facilitate drug testing, and advance research on overcoming chemotherapy resistance in OCCC.
OVMANA cells were originally isolated from ovary of a stage IV ovarian tumor patient.
The OVMANA cell line growth rate is poor after thawing, and many dead cells will be observed on the second day. Please try to seed the cells at a higer density. Once attached, the viable cells will grow with doubling time 2-3 days. Complete adherence to the protocol is necessary to ensure the viability of the cells.
1. The recommended culture medium for OVMANA is RPMI-1640 + 10%FBS.
Preheat the complete culture medium in a 37℃ water bath for 30 minutes.
Use a pipette to draw 6-7 mL of complete culture medium into a 15 mL centrifuge tube in a clean bench.
2. Then take the frozen cells out of the liquid nitrogen or dry ice. When reviving, shake them slightly to remove the residual dry ice or liquid nitrogen, then quickly clamp the lid with tweezers and place it in a 37℃ water bath and shake it quickly (note: the water cannot cover the lid), so that it completely melts in about 1 minute.
3. In the clean bench, wipe the outer wall of the cryotube with an 75% alcohol to disinfect it and let it dry slightly. Use a single-channel pipette to transfer all thawed cell suspensions to the complete culture medium prepared in advance, cover the lid, and centrifuge at 1100 rpm for 4 minutes at room temperature to collect cells.
4. Carefully aspirate the supernatant in the clean bench, use a single-channel pipette to absorb 1 mL of fresh complete culture medium to resuspend the cells into a single cell suspension, and then transfer to a T25 cm2 culture flask (or 6cm dish) containing 5 mL of complete culture medium.
Note: Do not directly recover to a T75 cm2 flask or a 10cm dish.
