OE-19 Luciferase Reporter Cell Line

Frequently Asked Questions

• What is the OE-19 Luciferase Reporter Cell Line?

  The OE-19 Luciferase Reporter Cell Line is generated from the OE-19 cell line, which originates from a human esophageal adenocarcinoma. This reporter cell line has been engineered to constitutively express the luciferase gene, allowing for bioluminescent tracking of cellular behaviors and responses in research.

• What are the growth conditions for the OE-19 Luciferase Reporter Cell Line?

  The OE-19 Luciferase Reporter Cell Line should be cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics-antimycotics. For maintaining selective pressure and ensuring stable luciferase expression, 0.5 µg/mL puromycin should be added to the culture medium.

• How should the OE-19 Luciferase Reporter Cell Line be thawed and processed upon receipt?

  Upon receipt, quickly thaw the frozen vials by agitating them in a 37°C water bath for about 2 minutes, ensuring the vial remains above the water surface to prevent contamination. After thawing, decontaminate the vial with 70% ethanol, transfer the contents into a centrifuge tube containing 5 mL of complete culture medium, centrifuge at 200×g for 5-10 minutes, discard the supernatant, and resuspend the cells in fresh medium before transferring them to a new culture flask.

• What are the specific applications of the OE-19 Luciferase Reporter Cell Line?

  The OE-19 Luciferase Reporter Cell Line is primarily utilized for studying gene expression and molecular pathways in esophageal adenocarcinoma. It facilitates the investigation of cancer biology, drug screening, and the efficacy of therapeutic interventions by providing a quantitative bioluminescent readout of cellular responses.

• What safety levels and precautions are associated with handling this cell line?

  The OE-19 Luciferase Reporter Cell Line is classified as biosafety level 1. Standard laboratory safety procedures should be followed, including wearing personal protective equipment and conducting all procedures in a biological safety cabinet to minimize the risk of contamination and ensure safe handling. The cells should only be used for in vitro experimental purposes and not for therapeutic or diagnostic use in humans or animals.