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NUGC-4, also referred to as ‘Nagoya University-Gastric Cancer-4’, ‘NU-GC-4’, and ‘NUGC4’, is a human gastric cancer cell line established from the patient during surgery. This cell line was derived from signet ring cell carcinoma of human stomach at the metastatic paragastric lymph nodes. When cultured in vitro, NUGC-4 cells 
exhibit a spherical epithelial-like morphology and display characteristics of cell adhension, although a few free-floating cells may be also present. On average, the doubling time of NUGC-4 is about 30 hours, and it should be cultured in PRMI1640 with 10% FBS.
Why choose NUGC-4 from AcceGen?
AcceGen supports NUGC-4 cells at more than 5×105 cells in 1mL volume with professional packaging of cryopreservation to ensure high quality and viability. Accegen NUGC-4 is negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. With the condition provided by AcceGen, the population doubling of NUGC-4 can reach more than 15 times.
| Product Code | NUGC4; NU-GC-4 |
| Species | Human |
| Cat.No | ABC-TC0862 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Lymph Node |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Gastric Cancer Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
As a cancer cell line, NUGC-4 is widely used in scientific research, such as searching for key factors of the formation of human gastric cancer and targets for anti-cancer drugs. Epidermal growth factor (EGF) strongly activates the tyrosine phosphorylation in NUGC-4. Phosphatidylinositol 3-kinase (PI 3-kinase), which is mainly activated by tyrosine kinases, was precipitated with anti-phosphotyrosine antibodies in NUGC-4. Exogenous hepatocyte growth factor can stimulate the proliferation of NUGC-4, and MET tyrosine kinase inhibitors inhibited this proliferation of NUGC-4. Investigation of NUGC-4 cell line and the interactions with growth factors may provide the direction for identifying the signaling pathway of gastric cancer and screening of potential therapeutic targets.
NUGC-4 cells are a human gastric cancer cell line derived from a poorly differentiated adenocarcinoma. They are widely used in cancer research, particularly for studies focused on gastric carcinoma, drug screening, and understanding cancer cell biology.
Slow Growth: Ensure regular medium change, check for contamination, and avoid over-confluency.
Poor Attachment: Use properly coated culture flasks and high-quality serum.
Cell Death: Maintain optimal culture conditions, verify reagents are non-toxic, and handle cells gently.
Cell Shape: NUGC-4 cells are epithelial-like in morphology. They typically appear as polygonal or slightly elongated cells when observed under a microscope.
Adherence: They adhere well to the surface of culture flasks, forming a cobblestone-like pattern characteristic of epithelial cells.
Cytoplasm and Nucleus: The cells have a well-defined cytoplasm and prominent nuclei, which are indicative of their active proliferative state.
