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NIH-3T3 | ||||
|---|---|---|---|---|
| Product Name | NIH-3T3 | |||
| Price | Get Quote | |||
| Product Code | NIH/3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3 | |||
| Cat.No | ABC-TC0828 | Species | Mouse | |
| Size/Quantity | 1 vial | Biosafety Level | 1 | |
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description |
Why choose NIH-3T3 from AcceGen? NIH-3T3 cells from AcceGen are characterized by high viability and quality. Rigorous quality control measures, including a PCR-based assay, guarantee the absence of mycoplasma. Daily monitoring ensures the cells remain free from bacteria, fungi, and yeast contamination. Additionally, the cell line’s authenticity is confirmed through STR analysis for precise cell identification. | |||
| Disease | Normal | |||
| Source Organ | Embryo | |||
| Recommended Medium And Supplement | DMEM + 10% FBS | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | FOR RESEARCH USE ONLY The NIH-3T3 cell line, a versatile fibroblast cell type, finds wide applications in DNA transfection studies and scientific research. Renowned for its spontaneous immortalization of mouse embryo cells, the 3T3 cell line serves as a valuable recipient in experiments involving the transfer of DNA from tumors. This application facilitates the discernment of transforming oncogenes, positioning NIH-3T3 cells as an indispensable tool for delving into the intricate molecular mechanisms governing cellular transformation and oncogenic processes in rigorous scientific investigations. | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | Fibroblast | |||
| Growth Mode | Adherent | |||
| Product Type | Mouse Embryo Cell Lines | |||
| Product Image |  | |||
NIH-3T3 cells, also recognized as Swiss 3T3, trace their origin to a mouse NIH/Swiss embryo and represent a fibroblast cell line characterized by distinctive attributes. Demonstrating heightened sensitivity to sarcoma virus focus formation and leukemia virus propagation, this multiclonal lineage manifests discernible clonal aberrations, housing a subclonal contingent predisposed to cellular transformation. Marked by perpetual proliferation in vitro and endowed with immortality, NIH-3T3 cells boast a doubling time of approximately 20 hours. When dispersed randomly on a chitosan surface, these cells assume a spindle-like morphology, while alignment on an OCMCS surface induces an elongated form. Despite an initial penchant for extreme contact inhibition, this hallmark has progressively waned within the cell line over successive passages.- ONLINE INQUIRY
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