NCI-H660 Luciferase Reporter Cell Line

Frequently Asked Questions

• What is the NCI-H660 Luciferase Reporter Cell Line?

  The NCI-H660 Luciferase Reporter Cell Line is generated from the NCI-H660 cell line, which originates from a prostate carcinoma in a 63-year-old male. This reporter line has been genetically modified to constitutively express the luciferase, allowing for bioluminescence-based assays that facilitate the study of gene expression, cellular responses to drugs, and signaling pathways in a cancer research context.

• Which promoter is the luciferase expression controlled by in the NCI-H660 Luciferase Reporter Cell Line?

  In the NCI-H660 Luciferase Reporter Cell Line, the expression of the luciferase gene is controlled by the EF1α (EFS) promoter. This promoter ensures stable and consistent expression of luciferase, making the cell line suitable for various experimental setups that require reliable reporter activity.

• What are the specific applications of the NCI-H660 Luciferase Reporter Cell Line?

  The NCI-H660 Luciferase Reporter Cell Line is primarily used for monitoring gene expression and cellular processes through luciferase assays. It is especially useful in cancer research, drug screening, and the study of gene regulation within the context of neuroendocrine carcinoma of the prostate. Researchers can use this cell line to quantify changes in luciferase activity in response to pharmaceutical treatments or genetic modifications.

• How should the NCI-H660 Luciferase Reporter Cell Line be cultured and maintained?

  The NCI-H660 Luciferase Reporter Cell Line should be cultured in RPMI 1640 medium supplemented with specific components including insulin, transferrin, sodium selenite, hydrocortisone, beta-estradiol, L-glutamine, and 5% fetal bovine serum. The cells exhibit mixed growth properties, with floating aggregates and some attached cells, and should be maintained at 37°C, 5% CO2. Medium renewal is recommended every 2 to 3 days.

• What are the storage and handling recommendations for the NCI-H660 Luciferase Reporter Cell Line?

  The NCI-H660 Luciferase Reporter Cell Line should be stored in the vapor phase of liquid nitrogen. Upon receipt, the cells should be thawed rapidly in a 37°C water bath and transferred to culture under strict aseptic conditions to ensure viability. It is crucial to handle the cells gently and avoid subjecting them to stress or contamination during the thawing and culturing processes.