NCI-H520 Luciferase Reporter Cell Line

Frequently Asked Questions

• What is the purpose of using NCI-H520 Luciferase Reporter Cell Line?

  The NCI-H520 Luciferase Reporter Cell Line is designed for research on lung squamous cell carcinoma. It allows researchers to monitor gene expression and study cellular responses by utilizing the luciferase reporter system, which provides a convenient and sensitive method for detecting luminescent signals.

• What are the recommended culture conditions for NCI-H520 Luciferase Reporter Cell Line?

  The recommended culture conditions include using RPMI 1640 medium supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin-streptomycin. The cells should be cultured at 37°C in a 5% CO₂ atmosphere.

• How should I thaw and handle the NCI-H520 Luciferase Reporter Cell Line upon receipt?

  Upon receiving the cryovial, place it in a 37°C water bath to thaw quickly. After thawing, transfer the cells into a tube with 5 mL of complete medium, centrifuge at 200×g for 5-10 minutes, and discard the supernatant. Resuspend the cells in fresh medium, and incubate them in a 37°C CO₂ incubator.

• What is the recommended subculturing process for NCI-H520 cells?

  When cells reach 80%-90% confluency, aspirate the medium, wash the cells with PBS, and treat them with 0.25% trypsin-EDTA to detach the cells. Neutralize the trypsin with complete medium, centrifuge the cells, and resuspend them in fresh medium. The recommended passage ratio is 1:2 to 1:4, with medium changes every 2 to 3 days.

• What quality control tests are performed on the NCI-H520 Luciferase Reporter Cell Line?

  The cell line undergoes stringent quality control, including tests for HIV-1, HBV, HCV, mycoplasma, fungi, yeast, and bacterial contamination, all of which must be negative. The viability of the cells is ensured to be over 90%, with a cell count of at least 1×10⁶ cells per vial.