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Nb2-11 | ||||
|---|---|---|---|---|
| Product Name | Nb2-11 | |||
| Price | Get Quote | |||
| Cat.No | ABC-TC5569 | Species | Rat | |
| Size/Quantity | 1 vial | |||
| Shipping Info | Dry ice | Storage | Liquid Nitrogen | |
| Description | Nb2-11 is a clone of the Nb-2 rat lymphoma line which was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Nb2-11 can also be mitogenically stimulated by IL-2. Injection of Nb2 cells into Nb rats gives rise to malignant tumours that are highly sensitive to treatment with vinca alkaloids. Karyotypic analysis has shown that the cell line has only five well developed chromosome abnormalities. | |||
| Source Organ | Thymus | |||
| Recommended Medium And Supplement | Fischer’s Medium + 10% FBS | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | For research use only | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | Lymphoblast | |||
| Growth Mode | Suspension | |||
| Product Type | Rat Lymphoma Cell Lines | |||
| Product Image |  | |||
Frequently Asked Questions
What is the growth mode of Nb2-11 cell line?
Nb2-11 cells are lymphoblastoid cells which grow in suspension and clump to form clusters when they are close to full confluence.
What culture medium is recommended for Nb2-11 cells?
The culture medium recommended for Nb2-11 cells is composed of Fischer’s medium supplemented with 0.075% Na Bicarbonate, 2mM Glutamine to support optimal cell growth and maintenance, add 0.05mM 2-Mercaptoethanol (2ME), 10% FBS and 10% HS. Nb2-11 cell culture does not have to use HEPEs.
What is the seeding density of Nb2-11 cell line?
It is recommended to seed at 4×1,000 and 12×1,000 cells/ml for culture periods of 96 and 72 hours respectively. Do not exceed 9×100,000 cells/ml. Check daily.
What are Nb2-11 cell line?
Nb2-11 is a clone of the Nb-2 rat lymphoma line, derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment.
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