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Mouse Splenocytes

Product Name

Mouse Splenocytes

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Cat.No

ABC-TC3937

Species

Mouse

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Mouse splenocytes (MS) are obtained from the spleen of healthy adult mice after the removal of splenic macrophages. The spleen serves as a hub for functions such as erythrocyte storage, hemoglobin metabolism, iron recycling, and importantly, playing a pivotal role in the immune system. By initiating primary immune responses to bloodborne antigens and producing antibodies, the spleen contributes significantly to immune defense. Mononuclear splenocytes encompass a spectrum of immune cells like T-lymphocytes, B-lymphocytes, NK cells, and NK T cells. Essential for immune research, primary MS can be utilized to isolate specific cell types such as CD4+ T-cells, CD8+ T-cells, and CD45R+ B cells.

 

Why choose Mouse Splenocytes from AcceGen?

Mouse splenocytes (MS), devoid of splenic macrophages, are isolated and cryopreserved, arriving frozen with a minimum of 10 million cells per 1mL vial. Rigorous quality control ensures viability and absence of contaminants such as mycoplasma, bacteria, yeast, and fungi. MS can be cultured temporarily using AcceGen’s specified conditions.

Disease

Normal

Source Organ

Spleen

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

FOR RESEARCH USE ONLY

Mouse splenocytes play a pivotal role in a wide array of immunology-based applications. These versatile cells are integral to various in-vitro immunoassays, including but not limited to ELISPOT, mixed lymphocyte reactions, and cytotoxicity assays measuring ADCC or CDC. Moreover, their significance extends to ex-vivo applications such as thorough cell population characterization and the generation of genetically modified cells through retroviral infection, highlighting their indispensable utility in advancing immunological research.

Growth Conditions

37 ℃, 5% CO2

Cell Type

Splenocyte

Growth Mode

Adherent

Product Type

Mouse Primary Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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