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MKN-1 | ||||
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| Product Name | MKN-1 | |||
| Price | Get Quote | |||
| Product Code | MKN-1; MKN 1 | |||
| Cat.No | ABC-TC0685 | Species | Human | |
| Size/Quantity | 1 vial | Biosafety Level | 1 | |
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description |
Why choose MKN-1 from AcceGen? Accegen offers every customer MKN-1 with the best quality and viability. The production of MKN-1 is conducted using standardized culture system and procedures. Before delivery, MKN-1 passes rigorous tests, including the parameters assessing cell morphology and viability, and negative performance for mycoplasma, bacteria, yeast, and fungi. | |||
| Source Organ | Stomach | |||
| Recommended Medium And Supplement | RPMI 1640 + 10% FBS | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | FOR RESEARCH USE ONLY Due to the rarity of human gastric adenosquamous carcinoma, limited clinical options are available to these patients. The utilization of MKN-1 in the scientific reports might unravel the cell proliferative properties of gastric carcinomas. MKN-1 uncovered that E-cadherin can be silenced by promoter CpG hypermethylation. MKN-1 can also used to discover inhibitory effects on tumor invasion. For example, eupatilin and exogenous RUNX3 has the potential to inhibit gastric cancer cell proliferation in MKN-1. | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Product Type | Human Gastric Cancer Cell Lines | |||
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MKN-1 is a human gastric adenosquamous carcinoma cell line derived from the metastatic site at the lymph node. MKN-1 has a point mutation amino acid replacements at exon5 of the p53. In 2023, there are about 26500 new cases of gastric cancer and 11130 deaths from this type of cancer in the USA. In all newly diagnosed cancer cases, gastric cancer is about 1.5%. Although gastric adenosquamous carcinoma is a rare type of gastric cancer with less than 1% incidence, it presents a more aggressiveness and serious prognosis compared with other types of gastric cancer.
Frequently Asked Questions
How do I maintain the morphology of MKN-1 cells?
Morphology: MKN-1 cells are epithelial in nature and should appear as adherent, polygonal cells. They should form a monolayer without significant clumping or detachment.
Monitoring: Regularly observe cells under a microscope to ensure they retain their characteristic morphology. Abnormalities in shape or detachment may indicate issues with culture conditions.
How do I manage cell doubling time?
Expected Doubling Time: MKN-1 cells typically have a doubling time of 24-36 hours under optimal conditions.
Troubleshooting Slow Growth
Nutrient Depletion: Ensure medium is changed every 2-3 days.
Cell Density: Avoid over-confluency. Split cells when they reach 70-80% confluence.
Contamination: Regularly check for microbial contamination.
Medium Quality: Use high-quality FBS and RPMI 1640 medium. Ensure they are not expired or contaminated.
Incubator Conditions: Confirm stable temperature and CO₂ levels in the incubator.How do I assess the health and viability of MKN-1 cells?
Viability Assays: Use trypan blue exclusion or MTT assay to determine cell viability.
Morphological Assessment: Regularly observe under a microscope for normal morphology.
Growth Curves: Plot cell growth over time to ensure consistent doubling time and healthy proliferation rates.
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