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| Species | Mouse |
| Cat.No | ABI-TC4178 |
| Product Category | Immortalized Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Macrophage |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Alveolus |
| Disease | Normal |
| Storage | Liquid Nitrogen |
| Product Type | Immortalized Cell Lines |
The MH-S cell line represents a continuous culture of murine alveolar macrophages (AM), initially derived from bronchoalveolar lavage cells of Balb/cJ mice via transformation with simian virus 40 (SV40). Characterized by their adhesion properties, lack of contact inhibition, and trypsin sensitivity, MH-S cells exhibit distinctive features. They express intracellular T-antigen and manifest DNA synthesis through the incorporation of 3H-thymidine, boasting a doubling time of approximately 48 hours, resulting in a doubling of cell numbers within 96 hours. MH-S cells maintain typical macrophage morphology, with over 98% esterase-positive cells, while being negative for peroxidase. They further express cell surface Ia and Mac-1 antigens and exhibit Fc receptor positivity, demonstrated by their capacity for rosette formation and phagocytosis of antibody-coated sheep erythrocytes. Importantly, akin to freshly isolated AM, MH-S cells display the ability to dose-dependently suppress the in vitro plaque-forming cell (PFC) response when co-cultured with splenic lymphocytes.
Why choose MH-S from AcceGen?
The MH-S cell line boasts exceptional features, including high viability and quality, meticulously maintained under optimal incubation conditions, cryopreserved by proficient operators, and delivered in a sterile state. Rigorous quality control ensures their accuracy and utility, making them a reliable choice for diverse research applications.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
The MH-S cell line, characterized by its preservation of typical alveolar macrophage morphology, holds substantial promise for applications in immunology research. This cell line is particularly valuable in investigations requiring homogeneous populations of alveolar macrophages. It provides a robust platform for elucidating the immunological functions of alveolar macrophages and their potential contributions to lung pathology, offering a critical tool for advancing our understanding of lung-related diseases and immune responses in this context.
The MH-S cell line is a macrophage, alveolar cell line, originally derived from the bronchoalveolar lavage of BALB/cJ mice and transformed with simian virus 40 (SV40).
These cells express cell surface Ia and Mac-1 antigens, are over 98% esterase-positive, negative for peroxidase, and exhibit Fc receptor positivity which supports their phagocytic activity.
MH-S cells are notable for their macrophage morphology, adherent and suspension growth properties, expression of intracellular T-antigen, and the ability to manifest DNA synthesis. They also display significant adhesion properties, lack of contact inhibition, and sensitivity to trypsin.
MH-S cells should be cultured in RPMI 1640 medium supplemented with 2mM Glutamine, 0.05mM 2-Mercaptoethanol, and 10% Fetal Bovine Serum (FBS).
MH-S cells are extensively used in immunology research, particularly for studies focusing on the role of alveolar macrophages in immune responses and lung pathology. They are crucial for experiments requiring homogeneous populations of alveolar macrophages.
