L6H2
1
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L6-H2 is a non-myogenic subclone of the rat myogenic L6 cell line, originally isolated from the skeletal muscle of newborn rats. These cells are maintained in continuous culture as a monolayer, characterized by a fibroblast-like appearance with lightly adherent round cells. Unlike their myogenic counterparts, L6-H2 cells do not exhibit myotube formation and can thrive in soft agar. For subculture, they are typically split at ratios of 1:10 to 1:20 using Trypsin/EDTA and seeded at a density of 2-4×104 cells per square centimeter.
Why choose L6-H2 from AcceGen?
L6-H2 cells from AcceGen exhibit exceptional features, boasting high viability and quality. These cells are validated for species authenticity through isoenzyme analysis, unequivocally confirming their rat origin with markers MD and NP. Furthermore, they undergo rigorous quality control measures, ensuring mycoplasma negativity and guaranteeing reliable and robust performance in research applications.
Product Code | L6-H2 |
Species | Rat |
Cat.No | ABC-TC0575 |
Product Category | Tumor Cell Lines |
Size/Quantity | 1 vial |
Shipping Info | Dry Ice |
Growth Conditions | 37 ℃, 5% CO2 |
Biosafety Level | 1 |
Storage | Liquid Nitrogen |
Product Type | Rat Fibroblas Lymphocytes Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
L6-H2 cells serve as a valuable tool in the realm of myogenic research, primarily functioning as a negative control within the context of L6 and its various subclones. They are instrumental in elucidating the characteristics of myogenic differentiation by providing a baseline for receptor expression, chemical stimulation, and signal transduction studies. By contrasting the behavior of L6-H2 cells with other variants, researchers can discern critical insights into the mechanisms governing muscle cell differentiation and further our understanding of this intricate process.