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J774A.1 Cells are active in antibody dependent phagocytosis [Pubmed: 1101071]. Their growth is inhibited by dextran sulfate, PPD and LPS. They synthesize large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocyt.
| Product Code | J-774A.1; J774.A1; J774 A1; J774A1; J 774A.1; J774 A.1 |
| Species | Mouse |
| Cat.No | ABC-TC0463 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Blood |
| Disease | Sarcoma |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Sarcoma Cell Lines |
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J774A.1 cells were isolated from the ascitic fluid of an adult female BALB/cN mouse with reticulum cell sarcoma. J774A.1 cells exhibit antibody-dependent phagocytosis and their growth is inhibited by dextran sulfate, PPD and LPS. J774A.1 cells can produce IL-1β and large amounts of lysozyme.
Cells are semi-adherent, subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Do not use trypsin. On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet, then re-suspended in fresh media. The J774A.1 seeding density is 2-4 x 10⁴/cm2, cells will show signs of re-attachment within 48 hours.
It seem so. The J774 mouse macrophages seem more susceptible to the toxic effects of oritavancin than human THP-1 cells. This is likely because J774 cells accumulate the drug to a greater extent than human cells.
