INS-1
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The INS-1 cell line, derived from x-ray-induced insulinoma in rats, exhibits a high insulin content and responsiveness to glucose changes. Cultivation relies on 2-mercaptoethanol and showcases gradual proliferation (100-hour doubling time) with beta-cell-like morphology. 
Heterogeneous in nature, INS-1 cells comprise mature insulin-positive and immature bi-hormonal cells co-expressing insulin and glucagon. Bi-hormonal variants, lower in Nkx6.1 and lacking alpha cell markers, imply incomplete maturation. Prolonged glucose exposure diminishes insulin gene and protein expression, subsequently reducing insulin and proglucagon-derived peptide levels, including GLP-1, GLP-2, and glucagon.
Why choose INS-1 from AcceGen?
INS-1 cells exhibit superior viability and quality, maintained under sterile conditions with optimal incubation settings. These cells are cryopreserved using advanced techniques and undergo rigorous quality control, ensuring their reliability for various experimental applications.
| Product Code | INS1 |
| Species | Rattus Norvegicus |
| Cat.No | ABC-TC232S |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | β Cell |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Pancreas |
| Storage | Liquid Nitrogen |
| Product Type | Rat Pancreas Cancer Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
The INS-1 cell line, renowned for its stability and preserved differentiation, serves as an ideal model to investigate diverse facets of beta-cell activity. It finds extensive utility in deciphering insulin secretion mechanisms, particularly excelling as a tool to explore glucose-triggered insulin release. Moreover, INS-1 cells offer valuable insights into the expression and regulation of proglucagon-derived peptides within primary islets, unraveling their responses to a range of metabolic stimuli.
The INS-1 cell line is an immortalized cells induced by radiation, isolated from pancreas of an x-ray-created transplantable insulinoma rats.
The culture medium recommended for INS- 1 cells is composed of 1640 medium supplemented with 4.5 g/L Glucose, 2 mM L-Glutamine, 10 mM HEPES, 1 mM Sodium pyruvate, 1.5 g/L NaHCO3 to support optimal cell growth and maintenance, add 10% FBS.
The sodium pyruvate, HEPES, and mercaptoethanol should be added at the specified concentration for cell growth.
The INS-1 cell line show weak attachment at the beginning of the recovery, usually take 24-48 hours without changing medium. In the first few days, observing floating cells is normal. This cell also grow slowly, may form attached clumps. Please be patient and take care. Normally 3 days after thawing, the cells can be trypsinized and plated on 2 flasks to expand.
