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Immortalized Mouse Kupffer Cell Line (ImKC)

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Immortalised Mouse Kupffer Cells (ImKC) are isolated from male H-2Kb-tsA58 transgenic mice which expresses the thermolabile mutant tsA58 of SV40 40 large T antigen and were sorted through identifying the F4/80 marker. The Immortalised cells expresses p53 levels with increases telomerase activity and retains the some functional features compared to the primary cells. ImKC cells […]
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Home | Immortalized Cell Lines | Immortalized Mouse Kupffer Cell Line (ImKC)
Species

Mouse
Cat.No

ABI-TC192D
Quality Control

All cells are tested negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Immortalized Cell Lines
Size/Quantity

1 vial
Cell Type

Kupffer Cell
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Liver
Disease

Normal
Biosafety Level

2
Storage

Liquid Nitrogen
Product Type

Immortalized Cell Line
Immortalization Method

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen.

Description

Immortalized Mouse Kupffer Cells (ImKCs) are selected from SV40 infected Mouse Kupffer cells (mKCs) which are isolated from C57BL/6 mouse liver tissue. ImKCs are cryopreserved with each vial containing more than 5×10^5 viable cells in 1ml volume. AcceGen offers optimized Mouse Kupffer Cell Medium for the expansion of ImKCs to give maximum performance. ImKCs are warranted with at least 10 passages if cultured following AcceGen’s detailed protocol.Immortalized Mouse Kupffer Cell Line can be used in standard biochemical procedures including PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.

AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

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