As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The Wild-type MutuDC1940 cell line is an Immortalised splenic CD8α+ subset (CD11chigh,B220-,DEC205+,CD24high,CD11b-) of conventional dendritic cells derived from the CD11c:SV40LgT transgenic mice. The MutuDC1940 cell line […]
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1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA, 4) Response to PAMP stimulation evaluated by functional assays.
Product Category
Immortalized Cell Lines
Size/Quantity
1 vial
Cell Type
DC Cell
Shipping Info
Dry Ice
Growth Conditions
37 ℃, 5% CO2
Source Organ
Lymphoid
Disease
Normal
Biosafety Level
2
Storage
Liquid Nitrogen
Product Type
Immortalized Cell Line
Immortalization Method
Immortalized Mouse Dendritic Cell Line was immortalized by transducing C57BL/6 mouse bone marrow isolates with retroviral vectors expressing GM-CSF, myc, and raf oncogenes.
Description
An immortalized mouse dendritic cell line refers to a type of cell line derived from mouse dendritic cells that have been modified to proliferate indefinitely in cell culture. Dendritic cells are a specialized type of immune cell responsible for presenting antigens to T cells, thereby initiating and regulating immune responses. Immortalized mouse dendritic cell lines are valuable tools in immunological research, as they provide a consistent and reproducible source of dendritic cells for studying various aspects of the immune system, including antigen presentation, immune activation, and tolerance mechanisms.
These cell lines are often generated through the introduction of genetic modifications or viral transformation to overcome the limited lifespan of primary dendritic cells in culture. Alternatively, dendritic cells can be fused with myeloma cells to create hybridomas that exhibit both the antigen-presenting capabilities of dendritic cells and the immortalized growth properties of myeloma cells.
Immortalized mouse dendritic cell lines offer several advantages for research purposes. They provide a consistent and homogeneous population of dendritic cells that can be easily expanded in culture, allowing for large-scale experiments and high-throughput screening assays. These cell lines also retain many of the functional characteristics of primary dendritic cells, including the ability to respond to microbial stimuli, present antigens to T cells, and regulate immune responses.
Why choose Immortalized Mouse Dendritic Cell Line from AcceGen?
Our stringent quality control measures guarantee the exceptional functionality of these dendritic cells:
Antigen Presentation Evaluation
Rigorous assessments of direct antigen presentation and cross-antigen presentation in MHC-I (SIINFEKL/OT-I) and MHC-II (OVA323-339/OT-II) restricted systems, ensuring accurate and efficient antigen processing.
Proteome Profiling and Surface Marker Assessment
Utilizing RT-PCR, we thoroughly examine the proteome profile and surface markers, providing insights into the cell line’s characteristics and ensuring reliability.
IL-12 Cytokine Secretion Analysis
Employing ELISA, we analyze IL-12 cytokine secretion, offering valuable information about the cell line’s immune response and functionality.
PAMP Stimulation Responsiveness
Functional assays evaluate the cell line’s response to PAMP stimulation, ensuring its suitability for various experimental conditions.
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Citation
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Application
FOR RESEARCH USE ONLY
AcceGen’s Immortalized Mouse Dendritic Cell Line serves as a valuable tool in various scientific research applications, particularly in the field of immunology. Some key applications include:
Immunology Research
The cell line is instrumental in studying immune responses, especially those related to dendritic cells—the key regulators of the immune system.
Antigen Presentation Studies
Researchers can utilize this cell line to investigate direct antigen presentation and cross-antigen presentation mechanisms, providing insights into how antigens are processed and presented to T cells.
Functional Assays
The cell line’s responsiveness to pathogen-associated molecular pattern (PAMP) stimulation allows researchers to conduct functional assays, contributing to a deeper understanding of cellular responses to various stimuli.
Cytokine Secretion Analysis
The analysis of cytokine secretion, particularly IL-12, provides crucial information about the cell line’s immune functionality and its role in modulating immune responses.
Proteome Profiling
By assessing the proteome profile and surface markers through RT-PCR, researchers gain a comprehensive understanding of the cell line’s characteristics, aiding in experimental design and data interpretation.
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