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Immortalized Human Lens Epithelial Cell Line – SV40 | ||||
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| Product Name | Immortalized Human Lens Epithelial Cell Line – SV40 | |||
| Price | Get Quote | |||
| Cat.No | ABI-TC170D | Species | Human | |
| Size/Quantity | 1 vial | Biosafety Level | 2 | |
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description | The Immortalised Lens Epithelial Cells – SV40 is established from primary cells (isolated from infant lens epithelial fragments) using Adl2-SV40 adenovirus. The Immortalised Human Lens Epithelial Cells – SV40 provides a homogeneous population of cell for the investigation of the physiology of the normal human lens epithelium and study its response to cataractogenic agents and the human lens specific functions. These cells also provide a unique system for examining the relationship between gene expression and differentiation. | |||
| Disease | Normal | |||
| Source Organ | Eye | |||
| Quality Control | The Immortalized Human Lens Epithelial Cells react positively with the monoclonal antibody to SV40 large T antigen. Western blot and precipitation are used to confirm the presence of βH- and γ-crystallin, the proteins that are markers for cell differentiation in the lens epithelium. SV40 was observed through IHC (Figure 6). | |||
| Recommended Medium And Supplement | ABI-TM170D Human Lens Epithelial Cell Line – SV40 Medium Kit | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | For research use only | |||
| Morphology | Polygonal | |||
| Immortalization Method | Serial passaging and transduction with recombinant adenovirus carrying SV40 Large T antigen | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | Epithelial | |||
| Growth Mode | Adherent | |||
| Product Type | Immortalized Cell Line | |||
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