The cell line was prepared by immortalizing hepatocytes from a 12 year-old Caucasian female donor with the SV40 large T antigen. The donor tested negative for CMV, HIV, HBV, and HCV.
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Immortalized Human Hepatocytes (IHH) are liver cells that have been genetically modified to extend their functional lifespan in culture, maintaining stable characteristics during short-term cultivation. These cells have shown promise in therapeutic applications, such as improving survival rates in preclinical models of acute liver failure when transplanted before the onset of severe damage.
IHH do not proliferate indefinitely, but they still offer significant value in liver disease research and potential therapeutic use due to their sustained functionality over short periods. Advances in cellular modification techniques, including telomerase activation via human telomerase reverse transcriptase (hTERT) and alterations in key regulatory pathways (such as p16/RB or p53), enhance the potential for their use in diverse applications. IHH are emerging as a critical resource in the development of cell-based therapies for liver-related disorders.
Why choose Immortalized human hepatocytes from AcceGen?
AcceGen pioneers the field of biomedical research with its cutting-edge immortalized human hepatocytes (IHH), meticulously crafted through advanced genetic modifications. Our IHH undergo rigorous quality control tests, ensuring the highest standards for your research needs:
Sterility Assurance
Rigorously tested for bacterial, fungal, Mycoplasma (PCR), and human pathogen contamination, guaranteeing pristine cell cultures.
Viability Excellence
Boasting over 95% post-thaw viability, our IHH exhibit robustness and resilience crucial for consistent and reliable experimental outcomes.
Identity Verification
Through Short Tandem Repeat (STR) analysis featuring 16 probes, including 15 STR loci and Amelogenin, we ascertain the precise genetic identity of our immortalized hepatocytes.
Growth Optimization
Evaluated based on morphology and immunocytochemistry of multiple undifferentiation markers, our IHH maintain characteristics akin to primary hepatocytes, ensuring authenticity in your studies.
Species
Human
Cat.No
ABI-TC3500
Product Category
Immortalized Cell Lines
Size/Quantity
1 vial
Cell Type
Hepatocyte
Shipping Info
Dry Ice
Growth Conditions
37 ℃, 5% CO2
Source Organ
Liver
Disease
Normal
Storage
Liquid Nitrogen
Product Type
Immortalized Cell Line
Citation
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
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Application
FOR RESEARCH USE ONLY
Immortalized human hepatocytes find diverse applications in scientific research, particularly in the field of hepatology and related areas. Some key applications include:
Biomedical Research
Immortalized human hepatocytes serve as crucial tools for studying liver-related diseases, enabling researchers to explore the molecular mechanisms underlying conditions such as hepatitis, cirrhosis, and liver cancers. These cells provide valuable insights into disease progression and potential therapeutic targets.
Drug Metabolism Studies
Researchers use immortalized human hepatocytes to assess drug metabolism and toxicity. These cells mimic the metabolic functions of primary hepatocytes, allowing for the evaluation of drug interactions, metabolite profiling, and the identification of potential adverse effects on the liver.
Cell-Based Therapies
The immortalization of hepatocytes contributes to the development of cell-based therapies for liver diseases. These cells can potentially be utilized for transplantation purposes, aiding in the regeneration of damaged liver tissue and offering novel therapeutic approaches for acute liver failure and other liver disorders.
Disease Modeling
Immortalized hepatocytes are employed to create in vitro models for various liver diseases. These models help researchers investigate the pathophysiology of diseases, test therapeutic interventions, and develop new treatment strategies.
Functional Studies
Researchers use immortalized hepatocytes to conduct functional studies related to liver physiology. This includes exploring signaling pathways, cytokine production, and responses to external stimuli, providing a comprehensive understanding of hepatocellular functions.
Viral Infection Studies
Immortalized hepatocytes are instrumental in studying viral infections affecting the liver, such as hepatitis viruses. Researchers can investigate the host-virus interactions, viral replication cycles, and the impact of antiviral drugs on infected hepatocytes.
Frequently Asked Questions
What are immortalized human hepatocytes?
Immortalized human hepatocytes are liver cells that have been genetically modified to proliferate indefinitely. This is typically achieved by introducing specific genes or using viral vectors that prevent the cells from undergoing senescence, thereby allowing continuous cell division and growth. These cells retain many of the functional characteristics of primary hepatocytes, making them valuable for research and therapeutic purposes.
How are immortalized human hepatocytes used in research?
Immortalized human hepatocytes are used extensively in research to study liver function, drug metabolism, and toxicity. They provide a stable and consistent model for investigating hepatic diseases, including hepatitis and liver cancer.
What are the benefits of using immortalized human hepatocytes?
Prolonged Lifespan: They can be cultured for extended periods, providing a consistent and renewable cell source.
Liver-Specific Functions: They retain many characteristics of primary hepatocytes, such as enzyme activity related to drug metabolism.
Reproducibility: Their continuous growth allows for reproducible experiments and large-scale studies.
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