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Immortalized Cell Lines

Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells

  • BSL

    1

  • 130
Human Bone Marrow-Derived Mesenchymal Stem Cells (HBMMSCs) are isolated from human Bone Marrow tissues and demonstrated with spindle-shaped-, and fibroblast-like cells. Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells are selected from Puromycin resistant HBMMSCs after infected with lentiviruses expressing SV40 LT under the control of TetON system.
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Immortalized Human Bone Marrow-Derived Mesenchymal Stem CellsHuman Bone Marrow-derived Mesenchymal Stem Cells (hMSCs) are highly promising for both clinical and experimental applications, owing to their ability to self-renew and differentiate into various mesenchymal lineages. These cells play a critical role in promoting the growth and differentiation of primitive hematopoietic cells, as well as maintaining leukemic cells. However, primary cultures of hMSCs suffer from limitations such as a restricted in vitro lifespan and variable properties among cells from different donors and over time in culture. To overcome these challenges, we have successfully generated immortalized cells by introducing a retrovirus carrying the SV40 large T antigen into primary mesenchymal cells, offering a renewable and consistent source of hMSCs for future therapeutic and research endeavors.

 

Why Choose Immortalized Human Bone Marrow Derived Mesenchymal Stem Cells from AcceGen?

AcceGen’s Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells are an ideal choice due to their high viability (>75%), guaranteed sterility without detectable bacteria, fungi, and mycoplasma, and stringent quality control measures. The use of Real-Time PCR to quantify SV40 gene expression ensures the reliability and authenticity of the immortalized cell line, making it a reliable and trustworthy option for research and experimental applications.

Species

Human

Cat.No

ABC-H0029X

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Product Category Immortalized Cell Lines
Size/Quantity

1 vial

Cell Type

Mesenchymal Stem Cell

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Bone Marrow

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Immortalized Cell Line

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

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Application

  • FOR RESEARCH USE ONLY

  • Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells (hMSCs) have versatile applications. These cells are valuable for studying the bone marrow microenvironment and understanding the environmental cues that regulate hematopoiesis. Additionally, they find utility in the fields of regenerative medicine, gene therapy, cell differentiation and gene regulation, as well as cell-based screening assays. Immortalized hMSCs serve as essential tools for various research areas with potential implications for therapeutic advancements.

Inquiring Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells

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High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research

Tags

  • Bone Marrow (115)
  • Human (1729)
  • Mesenchymal Stem Cell (63)
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