IM95

Frequently Asked Questions

• What are IM95 cells and their primary use in research?

  IM95 cells are a type of B-cell lymphoma cell line derived from a patient with immunoblastic lymphoma. They are used to study the molecular mechanisms underlying lymphoma development, progression, and response to treatment.

• What is the morphology Characteristics of IM95 cells?

  IM95 cells are generally medium to large in size with a rounded or irregular shape. They can appear as single cells or form clusters.

• How often should the medium be changed during IM95 culture?

  The frequency of medium changes for IM95 cells depends on several factors, including their growth rate, confluency, and the specific experimental requirements. Here are general guidelines for medium changes:

  Daily Feeding: It is typically recommended to change the medium daily for IM95 cells to maintain optimal growth conditions. This helps ensure a fresh supply of nutrients, remove waste products, and maintain pH balance in the culture.

  Cell Density and Confluency: If the cells reach high confluency (80-90%), they should be subcultured or the medium changed to prevent overgrowth, which can lead to differentiation or reduced viability.

  Experimental Needs: Adjust the frequency of medium changes based on the specific requirements of your experiments. For example, if you are conducting long-term experiments or treatments, more frequent medium changes might be necessary to maintain consistent conditions.

  Monitoring: Regularly monitor the pH of the medium and the appearance of the cells. If the medium becomes acidic or yellowish, or if the cells start detaching or showing signs of stress, consider increasing the frequency of medium changes.

  By maintaining a consistent schedule of medium changes, you can help ensure healthy growth and optimal experimental outcomes for IM95 cells in culture.