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HuP-T4 | ||||
|---|---|---|---|---|
| Product Name | HuP-T4 | |||
| Price | Get Quote | |||
| Product Code | HUP-T4; Hu-P-T4; HuPT4; HUPT4 | |||
| Cat.No | ABC-TC463S | Species | Human | |
| Size/Quantity | 1 vial | |||
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description | Tissue: pancreas; Tumor: adenocarcinoma; Derived from ascitic fluid. Karotype: modal number 77. CEA and CA19-9 production. | |||
| Source Organ | Pancreas | |||
| Recommended Medium And Supplement | EMEM + 10% FBS | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | For research use only | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | Epithelial | |||
| Growth Mode | Adherent | |||
| Product Type | Human Pancreas Cancer Cell Lines | |||
| Product Image |  | |||
Frequently Asked Questions
What is the HuP-T4 cell line?
HuP-T4 is a human pancreas cancer cell line derived from a patient with pancreatic adenocarcinoma. It was established from the ascitic fluid of the patient and is used to study pancreatic cancer biology.
What are the key characteristics of HuP-T4 cells?
HuP-T4 cells are epithelial and grow in an adherent manner. Their karyotype shows a modal chromosome number of 77. These cells are known to produce carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9), both of which are important tumor markers in pancreatic cancer.
What is the recommended medium for culturing HuP-T4 cells?
The recommended culture medium for HuP-T4 cells is 80% MEM (Minimum Essential Medium with Earle’s salts), supplemented with 20% heat-inactivated Fetal Bovine Serum (h.i. FBS), 1% non-essential amino acids, and 1% sodium pyruvate.
What biosafety level is required for handling HuP-T4 cells?
HuP-T4 cells require Biosafety Level 1 (BSL-1) precautions, as they are not known to pose significant health risks under normal laboratory conditions. However, standard safety protocols should be followed.
What is the mycoplasma status of the HuP-T4 cell line?
The HuP-T4 cell line has been tested for mycoplasma contamination and has been confirmed negative in tests using DAPI staining, microbiological culture, RNA hybridization, and PCR assays, ensuring its suitability for research.
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