Human Umbilical Vein Endothelial Cells (HUVECs) are cells from the vein of umbilical cord. They have cobblestone morphology with large dark nuclei when observed under light microscope. HUVECs are the most commonly used source of endothelial cells due to their easy accessibility and affordability in price.
How to obtain human umbilical vein endothelial cells
HUVEC cells can be obtained from discorded umbilical cord after childbirth by enzymatic digestion of the lumen. They express many important endothelial signal molecules, for example von Willebrand factor (vWF), which is associated with vascular homeostasis regulation, blood platelets mediation and blood vessel wall tone.
Why choose HUVECs from AcceGen
The isolation of human umbilical vein endothelial cells in AcceGen is from umbilical vein vascular wall by collagenase digestion and culture. Each vial contains 5*10^5 cells with high quality. Cells are cryopreserved at the end of primary culture in cell culture media kits with 10% DMSO and delivered frozen. HUVECs are guaranteed a further expansion of the population by 15 times when cultured with AcceGen recommended culture medium and culture protocols.
HUVECs are tested for specific antibodies against vWF/Factor VIII, CD31 and uptake of Dil-Ac-LDL by immunofluorescent approach. All cells lots are detected negative for the presence of HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
AcceGen recommends handling HUVECs with appropriate biosafety procedures due to its human origin. If not using HUVECs immediately, transfer cells to liquid nitrogen (-180) until ready for use.
