Trabecular meshwork cells (TMC) play an active role in the aqueous outflow mechanism. Specific receptors for neurotransmitters and neuropeptides, including epinephrine, acetylcholine, and neuropeptide Y, have been identified in TMC. In addition, a long list of vasoactive peptides and growth factors trigger intracellular signaling mechanisms in TM cells. TMC synthesize a great variety of extracellular matrix proteins as well as metelloproteinases. TMC culture has provided an invaluable tool to characterize pharmacological properties of TMC.TMC from AcceGen Research Laboratories are isolated from juxtacanalicular and corneoscleral region of the human eye. TMC are cryopreserved on primary culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HTMC are characterized by immunofluorescent method with antibodies to alpha-smooth muscle actin and fibronectin. TMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. TMC are guaranteed to further expand for 15 population doublings in the conditions specified by AcceGen Research Laboratories.
