Lining on the intestinal lumen surface, human small intestinal epithelial cells (IECs) play a crucial role in food digestion, nutrient absorption and more importantly, constitutes a physical barrier that separates host from commensal microbiota. The crypts and villi border the surface area of intestinal epithelium and homes a variety of specialized epithelial lineages including absorptive enterocytes, enteroendocrine cells, goblet cells and Paneth cells. Unique to small intestine, Paneth cells lines in the base of crypts and secretes numerous antimicrobial peptides (AMPs) that strictly regulate antimicrobial resistance. Deregulated intestinal barrier function is often associated with increased risk of intestinal inflammation, systematic immune activation, development of inflammatory bowel disease (IBD) and increased sensitivity to chronic viral infections.
Why choose Human small intestine epithelial cells from AcceGen?
AcceGen Human small intestine epithelial cells are isolated from whole small intestines and dissociated into single cells. Cells are cryopreserved at P2 immediately after isolation. Each vial contains at least 5×10^5 cells in 1 ml volume. Human Small Intestinal Epithelial Cells are tested for expression of markers using antibody, E-cadherin or ZO-1 Rabbit Polyclonal Antibody by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. Cells can be expanded for 2-4 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by AcceGen.
