Human Sebocytes

Cultured human sebocytes exhibit morphological changes during growth, transitioning from flat and elongated cells with round nuclei to larger, roundish cells with abundant cytoplasm and eccentric nuclei as secondary colonies develop. While maintaining key sebocytic traits, these cells undergo incomplete terminal differentiation in vitro. They synthetize fatty acids without bacterial involvement, express interleukin 1α, and respond to androgens in a location-dependent manner. Androgen-induced sebocyte proliferation is inhibited by spironolactone, and these cells demonstrate strong type 1 5α-reductase expression, metabolizing testosterone. Hormones like insulin, thyroid-stimulating hormone, and hydrocortisone stimulate proliferation, while retinoids inhibit proliferation, lipid synthesis, and differentiation, with isotretinoin as the most potent compound. Vitamin A proves crucial for sebocyte activity and differentiation, substitutable in part by synthetic retinoids.

Why choose Human Sebocytes from AcceGen?

Human Sebocytes from AcceGen are sourced from fully consented donors under IRB approval. These sebocytes are identified through Keratin-7 and MUC-1 staining as well as lipid accumulation assessment. With a purity exceeding 95%, the cultures are backed by optimized media for robust in vitro growth. These cells exhibit a consistent fibroblast morphology and boast high viability, guaranteeing reliability and suitability for various research applications.