Home > Products > Primary Cells > Human Primary Cells > Immune System Primary Cells >
Human Peripheral Blood Immature Dendritic Cells | ||||
|---|---|---|---|---|
| Product Name | Human Peripheral Blood Immature Dendritic Cells | |||
| Price | Get Quote | |||
| Cat.No | ABC-TC5467 | Species | Human | |
| Size/Quantity | 1 vial | |||
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description | Primary human immature dendritic cells (DCs) were derived from immunomagnetically selected peripheral blood (PB) monocytes. Monocytes were cultured in RPMI 1640 Medium with 10% fetal bovine serum (FBS), GM-CSF and IL-4 for 5 days to generate immature DCs. PB was collected using one of the following anticoagulants: acid-citrate-dextrose (ACD), acid-citrate-dextrose solution A (ACDA), citrate-phosphate-dextrose (CPD), citrate-phosphate-double-dextrose (CP2D), or citrate-phosphate-dextrose-adenine (CPDA). | |||
| Disease | Normal | |||
| Source Organ | Peripheral Blood | |||
| Recommended Medium And Supplement | ABM-TM5467 Human Peripheral Blood Immature Dendritic Cells Medium Kit | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | For research use only | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | DC Cell | |||
| Growth Mode | Suspension | |||
| Product Type | Immune Cells | |||
| Product Image |  | |||
Frequently Asked Questions
What are Human Peripheral Blood Immature Dendritic Cells?
Human Peripheral Blood Immature Dendritic Cells are antigen-presenting cells derived from peripheral blood monocytes. They play a crucial role in the immune system by capturing, processing, and presenting antigens to T cells. These cells are in an immature state, meaning they are highly phagocytic and have not yet fully developed the ability to stimulate T cells.
How are Immature Dendritic Cells obtained from peripheral blood?
Immature dendritic cells can be obtained from peripheral blood mononuclear cells (PBMCs) through a process that typically involves isolation of monocytes followed by their culture in the presence of specific cytokines, such as GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) and IL-4 (Interleukin-4), which promote differentiation into immature dendritic cells.
What are the key surface markers of Immature Dendritic Cells?
Immature dendritic cells typically express markers such as CD11c, HLA-DR, and CD14, but have low expression of co-stimulatory molecules like CD80, CD83, and CD86, which are upregulated upon maturation.
What are the differences between immature and mature dendritic cells?
Immature dendritic cells are primarily involved in antigen capture and processing, while mature dendritic cells have a reduced phagocytic ability and are more effective at presenting antigens to T cells, initiating an immune response. Maturation involves upregulation of co-stimulatory molecules and increased migration to lymphoid tissues.
How do you induce maturation of Immature Dendritic Cells in vitro
Maturation of immature dendritic cells can be induced in vitro by exposing them to various stimuli such as lipopolysaccharide (LPS), TNF-α (Tumor Necrosis Factor-alpha), or other pro-inflammatory cytokines. These agents trigger the cells to upregulate co-stimulatory molecules and become effective antigen-presenting cells.
- ONLINE INQUIRY
- PRODUCT REVIEWS
Fill out a request in the form below and we’ll get back to you within 24 hours with a quote.
