Human PD-1 (Luc) Jurkat Reporter Cell

The Human PD-1 (Luc) Jurkat Reporter Cell was engineered to not only express the NFAT response element driving luciferase expressing systems, but also express the receptor full length human PD-1 (Gene ID: 5133), which can use to evaluate the potency of PD-1 blockade. When cocultured with target cells expressing human PD-L1, the PD-1/PD-L1 interaction inhibits TCR signaling and NFAT-mediated luminescence. Blocking the PD-1/PD-L1 interaction by either anti-PD-1 or anti-PD-L1 antibodies releases the inhibitory signal and results in TCR activation and NFAT-mediated luminescence.