Human Nucleus Pulposus Cells

Human Nucleus Pulposus Cells (HNPCs) are isolated from the nucleus pulposus, the gel-like core within the intervertebral disc of the human spine. These cells are instrumental in the distribution of hydraulic pressure within the disc, a vital mechanism for resisting compressive loads and sustaining spinal flexibility. Nucleus pulposus cells reside in an environment characterized by a meager vascular supply, relying heavily on anaerobic glycolysis for energy production. Notably, their ability to adapt and thrive within this unique microenvironment is primarily attributed to the expression of two critical transcription factors, namely Hypoxia-Inducible Factor-1ɑ (HIF-1ɑ) and Tonicity-Responsive Enhancer Binding Protein (TonEBP/NFAT5). HNPCs are involved in synthesizing key structural proteins like collagen II and X, express hypoxia-inducible factors, and secrete various cytokines, including interleukins 1, 6, and 10, along with granulocyte-macrophage colony-stimulating factor. Furthermore, mechanical stress stimulates HNPC proliferation, underscoring their pivotal role in maintaining intervertebral disc homeostasis and function.

Why choose Human Nucleus Pulposus Cells from AcceGen?

AcceGen’s Human Nucleus Pulposus Cells (HNPC) are cryopreserved at passage one, delivered frozen, and each vial contains over 0.5×10⁶ cells in 1mL volume. They are characterized through immunofluorescence, demonstrating specific markers like fibronectin and vimentin. Importantly, HNPC provided by AcceGen are rigorously screened and found to be negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. Furthermore, these cells are guaranteed to undergo 15 population doublings under the recommended conditions, ensuring their robust expansion and usability in various applications.