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Human Nucleus Pulposus Cells

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Product Name

Human Nucleus Pulposus Cells

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Cat.No

ABC-TC3728

Species

Human

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Human Nucleus Pulposus Cells (HNPCs) are isolated from the nucleus pulposus, the gel-like core within the intervertebral disc of the human spine. These cells are instrumental in the distribution of hydraulic pressure within the disc, a vital mechanism for resisting compressive loads and sustaining spinal flexibility. Nucleus pulposus cells reside in an environment characterized by a meager vascular supply, relying heavily on anaerobic glycolysis for energy production. Notably, their ability to adapt and thrive within this unique microenvironment is primarily attributed to the expression of two critical transcription factors, namely Hypoxia-Inducible Factor-1ɑ (HIF-1ɑ) and Tonicity-Responsive Enhancer Binding Protein (TonEBP/NFAT5). HNPCs are involved in synthesizing key structural proteins like collagen II and X, express hypoxia-inducible factors, and secrete various cytokines, including interleukins 1, 6, and 10, along with granulocyte-macrophage colony-stimulating factor. Furthermore, mechanical stress stimulates HNPC proliferation, underscoring their pivotal role in maintaining intervertebral disc homeostasis and function.

 

Why choose Human Nucleus Pulposus Cells from AcceGen?

AcceGen’s Human Nucleus Pulposus Cells (HNPC) are cryopreserved at passage one, delivered frozen, and each vial contains over 0.5×106 cells in 1mL volume. They are characterized through immunofluorescence, demonstrating specific markers like fibronectin and vimentin. Importantly, HNPC provided by AcceGen are rigorously screened and found to be negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. Furthermore, these cells are guaranteed to undergo 15 population doublings under the recommended conditions, ensuring their robust expansion and usability in various applications.

Disease

Normal

Source Organ

Nucleus Pulposus

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

FOR RESEARCH USE ONLY

Human Nucleus Pulposus Cells (HNPCs) are versatile entities with a wide array of applications in spinal research. They provide a valuable in vitro model for the exploration of cellular and molecular mechanisms underpinning disc degeneration, offering critical insights into tissue engineering and cell-based therapeutic strategies for spinal disc disorders. Moreover, HNPCs are instrumental in characterizing the temporal response of pro-inflammatory cytokines released by HNPCs upon TNFα stimulation, aiding in the assessment of multiple anti-inflammatory agents’ efficacy. Additionally, these cells play a pivotal role in establishing sophisticated in vitro disease models, thereby enabling High Throughput and High Content Screening methodologies for the systematic evaluation of potential therapeutic interventions in the realm of spinal disc pathologies.

Growth Conditions

37 ℃, 5% CO2

Cell Type

Nucleus Pulposus Cell

Growth Mode

Adherent

Product Type

Cartilage Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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