Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts
BSL
102
Carcinoma-associated fibroblasts (CAFs) have recently been implicated in important aspects of epithelial solid tumor biology such as neoplastic progression, tumor growth, angiogenesis, and metastasis. Immunophenotypes of CAFs are different from Adenocarcinomas in Lung Cancer. These CAFs have therefore have separate and important prognostic value.Product is manufactured, tested and validated in an ISO 9001/ISO13485/CLIA certified facility.
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Human Lung Adenocarcinoma Cancer-Associated Fibroblasts (CAFs) are specialized cells isolated from human lung tumor tissue. CAFs play a significant role in the tumor microenvironment and have been implicated in various aspects of solid tumor biology, including neoplastic progression, tumor growth, angiogenesis, and metastasis.
These fibroblasts are specifically associated with lung adenocarcinoma, a type of non-small cell lung cancer that originates in the peripheral lung tissues. The term “cancer-associated fibroblasts” refers to fibroblastic cells found in the tumor stroma that interact with cancer cells and contribute to the complex dynamics of tumor development.
Why choose Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts from AcceGen?
Introducing our meticulously crafted product, manufactured, tested, and validated in an ISO 9001/ISO13485/CLIA certified facility, ensuring the highest standards of quality and safety. Our commitment to excellence is demonstrated through a rigorous set of quality control (QC) tests performed on each batch:
Sterility
We conduct comprehensive tests to ensure a negative result for contamination, covering bacterial, fungal, and Mycoplasma (PCR). Additionally, our product is confirmed negative for human pathogens, meeting the stringent requirements for purity.
Viability
Post-thaw viability is a crucial aspect of cell functionality. Our product consistently achieves a viability rate of over 95%, ensuring the cells’ robustness and effectiveness in various applications.
Identity
Utilizing Short Tandem Repeat (STR) analysis, we verify the identity of our product with precision. This involves checking 16 probes, including 15 STR loci and Amelogenin, guaranteeing the authenticity and uniqueness of the cell line.
Growth
We assess the growth characteristics through detailed examinations of morphology and immunocytochemistry involving multiple undifferentiation markers. This ensures the reliability and stability of the cell line throughout its life cycle.
Species
Human
Cat.No
ABC-TC5539
Product Category
Primary Cells
Size/Quantity
1 vial
Cell Type
Fibroblast
Shipping Info
Dry ice
Growth Conditions
37 ℃, 5% CO2
Source Organ
Lung
Disease
Lung Adenocarcinoma Carcinoma
Storage
Liquid Nitrogen
Product Type
Human Primary Carcinoma Associated Fibroblasts
Citation
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Application
FOR RESEARCH USE ONLY
The Human Lung Adenocarcinoma Cancer-Associated Fibroblasts (CAFs) offer a versatile platform for a range of research applications. Key applications include:
Cell-Cell Interaction Studies
Human Lung Adenocarcinoma CAFs are suitable for exploring cell-cell interactions, providing valuable insights into the dynamic processes within the tumor microenvironment.
Adhesion Assays
Researchers can use these cells to investigate adhesion mechanisms, contributing to a better understanding of cancer cell behavior and potential therapeutic targets.
PCR (Polymerase Chain Reaction)
The cells are amenable to PCR analysis, enabling the study of specific gene expression patterns or molecular changes associated with lung adenocarcinoma.
Western Blotting
Human Lung Adenocarcinoma CAFs serve as a reliable source for protein analysis through Western blotting, aiding in the identification and quantification of specific proteins.
Immunoprecipitation
The cells can be utilized for immunoprecipitation studies, allowing researchers to isolate and analyze specific proteins or protein complexes.
Immunofluorescent Flow Cytometry
These CAFs are suitable for immunofluorescent flow cytometry, enabling the assessment of cell surface markers and intracellular proteins, providing a deeper understanding of cellular characteristics.
Cell Derivative Generation
Researchers can generate cell derivatives from these CAFs for customized research applications, expanding the versatility of the cell line.
Frequently Asked Questions
What are Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts (CAFs)?
Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts (CAFs) are a type of fibroblast found in the tumor microenvironment of lung adenocarcinoma. They play a significant role in supporting tumor growth, invasion, and metastasis by interacting with cancer cells and modifying the extracellular matrix.
How are Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts different?
Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts (CAFs) differ from normal fibroblasts in that they are activated and often exhibit altered gene expression, enhanced secretion of growth factors, cytokines, and proteases. These changes enable them to support tumor progression, whereas normal fibroblasts typically function in tissue repair and maintenance.
What role do Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts play?
In lung adenocarcinoma, Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts contribute to tumor growth and metastasis by promoting angiogenesis, suppressing immune responses, remodeling the extracellular matrix, and facilitating cancer cell invasion and migration. They are key players in creating a tumor-promoting environment.
How are Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts identified?
Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts are identified by their expression of specific markers such as alpha-smooth muscle actin (α-SMA), fibroblast activation protein (FAP), and platelet-derived growth factor receptor (PDGFR). These markers help distinguish them from normal fibroblasts and other cell types in the tumor microenvironment.
Can Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts be targeted for cancer therapy?
Yes, targeting Human Lung Adenocarcinoma Carcinoma Associated Fibroblasts is an emerging strategy in cancer therapy. Since CAFs play a crucial role in supporting tumor growth and resistance to therapy, disrupting their function or altering their interactions with cancer cells could enhance the effectiveness of existing treatments and reduce tumor progression.
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