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Human Liver Sinusoidal Endothelial Cells

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Product Name

Human Liver Sinusoidal Endothelial Cells

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Product Code

LSECs

Cat.No

ABC-TC3679

Species

Human

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Human Liver Sinusoidal Endothelial Cells (HLSECs) are the most abundant non-parenchymal hepatic cells (about 60%), strategically placed lining the hepatic sinusoid, and therefore become the first contact of tissue with circulating lymphocytes. Apart from forming a barrier within the hepatic sinusoids, more physiological and immunological functions of HLSECs have been discovered.
Liver Sinusoidal Endothelial Cells Function
The functions like filtration, endocytosis, antigen presentation and leukocyte recruitment. Compared to other capillary endothelial cells, liver sinusoidal cells exhibit unique structural and functional features – work as dendritic cells and present antigen for CD4+ T cells. The HLSEC maintain immune homeostasis within the liver, implicated in liver regeneration and mediate the immune response with acute and chronic liver injury.
Why Choose Human Liver Sinusoidal Endothelial Cells from AcceGen?
AcceGen cryopreserves primary human liver sinusoidal endothelial cells immediately after purification and delivered frozen. Each vial contains about 5 x 10^5 cells in 1 ml. AcceGen HLSECs are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM).

Disease

Normal

Source Organ

Liver

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement

ABM-TM3679 Human Liver Sinusoidal Endothelial Cells Medium Kit

Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

For research use only
Human hepatic sinusoidal endothelial cells are ideal in studies of chronic liver injury, toxicity, immune response, portal vein fibrosis, angiogenesis etc.

Key Features

-Backed by AcceGen advanced technology
-Cryopreserved for highest viability and plating efficiency
-Quality-tested for accurate results

Growth Conditions

37 ℃, 5% CO2

Cell Type

Endothelial

Growth Mode

Adherent

Product Type

Hepatic Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial

Frequently Asked Questions

  • What are the recommended culture conditions for Human Liver Sinusoidal Endothelial Cells?

    These cells should be cultured in endothelial cell growth medium, supplemented with specialized growth factors.

  • Are these cells cryopreserved, and what is the viability after thawing the cells?

    Yes, they are cryopreserved. Post-thaw viability is typically above 80%, if proper protocols are followed.

  • Can Human Liver Sinusoidal Endothelial Cells be used for drug screening or toxicity studies?

    Yes, these cells are suitable for in vitro drug screening and toxicity studies related to liver function.

  • What is the typical morphology of Human Liver Sinusoidal Endothelial Cells in culture?

    These cells exhibit a typical cobblestone-like morphology of endothelial cells when cultured properly.

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