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Human LDLR knockout cell line

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Human LDLR knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.
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Home | Transfected Stable Cell Lines | Knockout Stable Cell Lines | Human LDLR knockout cell line
Species

Human
Cat.No

ABC-KH8365
Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Knockout Stable Cell Line
Host Cell

HEK293; HeLa or other cell lines

Description

Human LDLR knockout cell line is edited by CRISPR/Cas9 technology.

AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

Frequently Asked Questions

  • What is the objective of the Human LDLR Knockout in HEK293 project?

    The objective is to create a human LDLR knockout cell line using CRISPR/Cas-mediated genome engineering. The knockout model can be used for studying LDLR’s role in cholesterol metabolism and other related cellular functions.

  • Which exon was selected as the target site for the knockout?

    Exon 2 of the LDLR gene was selected as the target site for the knockout. This exon contains important coding regions, making it an effective target for disrupting the LDLR function.

  • What methods are used to confirm knockout of the LDLR gene?

    PCR and sequencing are used to confirm the knockout of the LDLR gene. Clones are screened, and the regions containing the mutation are sequenced to verify the genotype.

  • How is the LDLR knockout cell line generated?

    The LDLR knockout cell line is generated through CRISPR/Cas-mediated genome engineering. Single clones are isolated and screened, followed by genotyping. Successful knockout clones are cryopreserved.

  • What are the storage and culture conditions for the Human LDLR Knockout HEK293 cells?

    The cells are stored in liquid nitrogen vapor phase. The recommended culture conditions include 95% air and 5% CO₂ at 37°C. The cells are cultured in basal medium, supplemented with 10% Fetal Bovine Serum (FBS).

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