Human Kupffer Cells

Frequently Asked Questions

• What are Kupffer cells?

  Kupffer cells are specialized macrophages located in the liver. They are part of the mononuclear phagocyte system and play a critical role in the body’s immune response by clearing pathogens, debris, and aged red blood cells from the blood.

• What is the primary function of Kupffer cells?

  The primary function of Kupffer cells is to act as scavengers in the liver, engulfing and digesting cellular debris, pathogens, and other particles through phagocytosis. They also produce cytokines and other molecules that modulate immune responses and inflammation.

• How to isolate Kupffer cells from liver?

  Kupffer cells are typically isolated from liver tissue through a process that involves enzymatic digestion of the liver, followed by density gradient centrifugation or magnetic sorting to separate them from other liver cell types.

• What roles do Kupffer cells play in liver homeostasis and metabolism?

  Kupffer cells contribute to liver homeostasis by clearing pathogens and debris, regulating immune responses, and producing cytokines that maintain the balance of liver cell functions. They also play a role in the metabolism of lipids and iron.

• How do Kupffer cells interact with other cells in the liver?

  Kupffer cells interact with hepatocytes, stellate cells, endothelial cells, and infiltrating immune cells through direct cell-to-cell contact and the secretion of signaling molecules. These interactions help coordinate immune responses and maintain liver function.