Human Hepatic Stellate Cells

Human Hepatic Stellate Cells (HHSC) are primary cells derived from the human liver and cultivated in optimized medium. Positioned between hepatocytes and blood vessels in the liver, they exhibit myofibroblast-like or lipocyte characteristics. HHSC play a crucial role in maintaining liver extracellular matrix homeostasis, contributing to repair, regeneration, fibrosis, and controlling retinol metabolism. Notably, they become activated into fibrogenic myofibroblast-like cells in conditions like chronic alcoholism, hepatitis B and C, fatty liver disease, obesity, and diabetes, leading to liver fibrosis. HHSC are also involved in regulating hepatic microcirculation and can contribute to intrahepatic portal hypertension in disease states. Their proliferation, migration, and chemokine expression are associated with liver inflammation and fibrogenesis.

Why choose Human Hepatic Stellate Cells from AcceGen?

AcceGen’s Human Hepatic Stellate Cells (HHSC) are cryopreserved at passage one and shipped in a frozen state, with each vial containing over 5×10⁵ cells in 1mL. These cells are characterized using immunofluorescence with α-smooth muscle actin-specific antibodies. They are thoroughly tested and confirmed to be negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. Furthermore, these HHSC are guaranteed to expand for up to 15 population doublings when cultured under the conditions provided by AcceGen.