Human FBXW7 knockout cell line

Frequently Asked Questions

• What is the Human FBXW7 knockout cell line?

  The Human FBXW7 knockout cell line is a genetically modified cell line in which the FBXW7 gene has been disrupted or “knocked out” using CRISPR/Cas9 technology. This allows researchers to study the function and role of the FBXW7 gene in cellular processes without its influence.

• Which host cells are used for creating the Human FBXW7 knockout cell line?

  The Human FBXW7 knockout cell line can be created using various host cells, including HEK293 and HeLa cells, among others. These cell lines are commonly used in research due to their robustness and ease of genetic manipulation.

• What applications are there for the Human FBXW7 knockout cell line?

  The Human FBXW7 knockout cell line is used in a variety of research applications, including:
  Studying the role of FBXW7 in cell cycle regulation and cancer;
  Investigating the molecular mechanisms of FBXW7-mediated protein degradation;
  Screening for potential therapeutic targets and drug development;
  Understanding the pathways and processes influenced by FBXW7 in different cellular contexts.

• How is the FBXW7 gene knocked out in these cell lines?

  The FBXW7 gene is knocked out using CRISPR/Cas9 technology. This involves designing a specific guide RNA (gRNA) that targets the FBXW7 gene. The Cas9 enzyme, guided by the gRNA, introduces double-strand breaks in the DNA at the target site. The cell’s repair mechanisms then lead to insertions or deletions (indels) that disrupt the gene’s function.

• What are the benefits of using the Human FBXW7 knockout cell line in research?

  The benefits of using the Human FBXW7 knockout cell line include:
  Ability to directly study the effects of FBXW7 loss of function;
  Enhanced understanding of FBXW7’s role in various biological processes and diseases, particularly cancer;
  Facilitates the identification of downstream targets and pathways regulated by FBXW7;
  Provides a valuable tool for drug screening and development by revealing potential therapeutic targets.