Human Endometriotic Stromal Cells

Frequently Asked Questions

• What are Human Endometriotic Stromal Cells?

  Human Endometriotic Stromal Cells are derived from the stromal tissue of endometriotic lesions. These cells are isolated from surgically obtained endometriotic tissue samples, ensuring high purity and viability for research applications.

• What are the primary applications of Human Endometriotic Stromal Cells in research?

  These cells are extensively used in studies focusing on endometriosis pathogenesis, drug screening, and the development of novel therapeutic strategies. They are also valuable for investigating the cellular and molecular mechanisms underlying endometriotic lesion formation and progression.

• What are the optimal growth conditions for culturing Human Endometriotic Stromal Cells?

  Human Endometriotic Stromal Cells require a specialized growth medium designed for stromal cells, typically supplemented with fetal bovine serum, growth factors, and other essential nutrients. Cells should be cultured at 37°C in a humidified atmosphere with 5% CO2.

• What are the key characteristics and markers of Human Endometriotic Stromal Cells?

  These cells exhibit fibroblast-like morphology and express specific markers such as vimentin, CD10, and estrogen receptors. They are also characterized by their ability to respond to hormonal stimuli, which is crucial for studying endometriosis.

• How should Human Endometriotic Stromal Cells be handled during passaging to ensure optimal cell state?

  When passaging these cells, it is important to avoid over-confluency, as it can affect their growth and viability. Typically, cells should be passaged at 70-80% confluency using standard trypsinization protocols. Ensuring gentle handling and using appropriate cell culture reagents will help maintain cell integrity and functionality.