Human dopaminergic neuronal cells are isolated from fetal brain tissue obtained from agencies authorized to procure and distribute tissues for research. Dopaminergic neuronal cells are selected in tyrosine free medium supplemented with a mixture of growth factors. Actively growing population of cells are tested for tyrosine hydroxylase (TH) expression by immunocytochemistry. HDaNCs cells are provided […]
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Human Dopaminergic Neuronal Precursor Cells (HDNPC) are isolated from human healthy brain tissue. These cells are cryopreserved at passage one and delivered frozen, with each vial containing over 500,000 viable cells. They are guaranteed to expand for up to 5 population doublings when following the instructions provided in the technical sheet.
Dopamine, a neurotransmitter extensively studied for its role in various mental and neurological disorders, is primarily produced by midbrain dopaminergic neurons within the mammalian central nervous system. Originally, the identification and localization of these neurons were achieved using the Falck-Hillarp histofluorescence method, which involves the visualization of fluorescent monoamines post-formaldehyde treatment .
Dopaminergic neurons are a heterogeneous group both anatomically and functionally, found in regions such as the diencephalon, mesencephalon, and olfactory bulb (Björklund & Lindvall, in press). The largest cluster of dopaminergic cells is situated in the ventral mesencephalon, constituting approximately 90% of the total dopaminergic cell population in the brain.
Why choose Human Dopaminergic Neuron Cells from AcceGen?
At AcceGen, we cryopreserve these dopaminergic neuron cells at passage 1 in specialized cell culture media, ensuring their preservation in peak condition. Following AcceGen’s provided procedures, the cells can be seamlessly recovered in neuron maintenance media, with a guaranteed expansion capability of up to 4 passages. Rest easy knowing that all our cells are rigorously tested and confirmed negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
Experience an additional dimension with our RFP-Human Dopaminergic Neuron Cells, exclusively available at AcceGen. Every cell undergoes rigorous testing for mycoplasma, bacteria, yeast, and fungi negativity, ensuring a superior standard of quality.
Key Features:
Backed by AcceGen’s cutting-edge technology
Cryopreserved to ensure maximum viability and plating efficiency
Quality-tested to deliver precise and reliable results
Product Code
HDaNCs
Species
Human
Cat.No
ABC-TC5463
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category
Primary Cells
Size/Quantity
1 vial
Cell Type
Neuron
Shipping Info
Dry Ice
Growth Conditions
37 ℃, 5% CO2
Disease
Normal
Biosafety Level
1
Storage
Liquid Nitrogen
Product Type
Nervous Cells
Citation
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Application
FOR RESEARCH USE ONLY
Some key applications include:
Neurodegenerative Disease Studies
Dopaminergic neurons are critical for studying neurodegenerative diseases such as Parkinson’s disease. Researchers use these cells to investigate disease mechanisms, test potential therapeutic interventions, and explore ways to protect or regenerate dopaminergic neurons.
Drug Discovery and Development
These cells play a crucial role in drug screening and testing. Pharmaceutical companies utilize dopaminergic neurons to assess the effects of new drugs on neuronal function and viability, especially those targeting neurological disorders.
Cellular and Molecular Neuroscience Research
Studying the properties and behavior of dopaminergic neurons provides insights into basic cellular and molecular neuroscience. This includes understanding neuronal development, neurotransmitter release, and the impact of various factors on neuronal function.
Parkinson’s Disease Modeling
Given the involvement of dopaminergic neurons in Parkinson’s disease, researchers use these cells to create in vitro models of the disease. This allows them to mimic pathological conditions, test hypotheses, and identify potential therapeutic targets.
Toxicology Studies
Dopaminergic neurons are employed in toxicology studies to assess the neurotoxic effects of various compounds. This is crucial for evaluating potential risks and ensuring the safety of chemicals and pharmaceuticals.
Cell Therapy and Regenerative Medicine
Understanding the biology of dopaminergic neurons is essential for the development of cell-based therapies for neurodegenerative disorders. Researchers explore ways to replace damaged or lost neurons with healthy dopaminergic cells.
Disease Mechanism Elucidation
Investigating the function and dysfunction of dopaminergic neurons helps elucidate the underlying mechanisms of various neurological disorders, contributing to a deeper understanding of brain function.
Frequently Asked Questions
What are human dopaminergic neurons, and what is their primary function?
Human dopaminergic neurons are neurons that produce and release dopamine, a neurotransmitter crucial for motor control, reward, and motivation. They play a key role in the central nervous system.
How are human dopaminergic neurons typically obtained and cultured for research purposes?
They are typically derived from stem cells, such as iPSCs or embryonic stem cells, through differentiation protocols. They are cultured in specialized media that support their growth and functionality
What are the main applications of Human Dopaminergic Neurons in scientific research?
They are used to study neurodegenerative diseases like Parkinson’s, for drug screening, and to understand dopamine-related disorders. Additionally, they assist in developing and testing new therapies.
What are the key characteristics when culturing Human Dopaminergic Neurons?
Key characteristics include the expression of markers like TH (tyrosine hydroxylase), DAT (dopamine transporter), and their ability to produce and release dopamine
What are the main challenges of culturing and experimenting with Human Dopaminergic Neurons?
Challenges include maintaining cell viability and dopaminergic properties, preventing contamination, and replicating the complex in vivo environment of the brain in vitro.
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