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Human Dermal Fibroblasts, Adult | ||||
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| Product Name | Human Dermal Fibroblasts, Adult | |||
| Price | Get Quote | |||
| Product Code | HDF-a | |||
| Cat.No | ABC-TC3029 | Species | Human | |
| Size/Quantity | 1 vial | Biosafety Level | 1 | |
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description | Derived from adult skin’s dermal layer, Human Dermal Fibroblasts, or HDFa, are instrumental in synthesizing the skin’s connective tissue matrix. These fibroblasts are pivotal players in wound healing, where they utilize Fibronectin (FN) to facilitate their migration. Beyond wound repair, they engage in intricate interactions with epidermal cells during hair development and interfollicular skin processes.
Why choose Human Dermal Fibroblasts, Adult from AcceGen? Human Dermal Fibroblasts, Adult (HDFa), are cryopreserved at passage one and supplied frozen, with each vial containing over 5×105 cells in 1 ml. They exhibit spindle morphology and fibronectin-specific immunofluorescence. Tested negative for various pathogens, including HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi, they offer safety assurance. HDFa can reliably expand for up to 15 population doublings under AcceGen’s specified conditions.
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| Disease | Normal | |||
| Source Organ | Dermal | |||
| Quality Control | All cells test negative for mycoplasma, bacteria, yeast, and fungi. | |||
| Recommended Medium And Supplement | ABM-TM3029 Human Dermal Fibroblasts, Adult Medium Kit | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | FOR RESEARCH USE ONLY HDFa serve as a versatile model system for investigating various facets of cell physiology and have been prominently featured in numerous research works, especially within the realms of skin biology and reprogramming studies. These fibroblasts find application in diverse areas such as pathogen response, skin aging, wound healing, gene delivery, and skin-related disorders like scleroderma. Their utility extends to research involving ECM protein analysis, collagen metabolism, cosmetics, skin therapy, and model development, encompassing an array of multidisciplinary research avenues. | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | Fibroblast | |||
| Growth Mode | Adherent | |||
| Product Type | Dermal Cells | |||
| Product Image |  | |||
Noteworthy is their pivotal role in the groundbreaking generation of induced pluripotent stem cells (iPSC) alongside synovocytes. This achievement, made possible by the “Yamanaka cocktail” containing Oct3/4, Sox2, Klf4, and c-Myc, garnered Dr. Shinya Yamanaka the 2012 Nobel Prize in recognition of its transformative impact.Frequently Asked Questions
What are Human Dermal Fibroblasts (HDFs)?
Human Dermal Fibroblasts (HDFs) are cells derived from the dermis layer of human skin. They play a crucial role in the synthesis and remodeling of the extracellular matrix and are essential for wound healing and skin maintenance.
How do I ensure the optimal growth and health of HDFs in culture?
To ensure optimal growth and health of HDFs, monitor cell confluency regularly and passage cells when they reach 80-90% confluency. Avoid over-confluency to prevent cell differentiation and senescence. Handle cells gently to avoid mechanical stress and maintain consistent culture conditions.
What are the morphological characteristics of HDFs in culture?
In culture, HDFs typically exhibit a spindle-shaped, elongated morphology with prominent nuclei. They tend to grow in a monolayer with characteristic fibroblastic extensions. The cells should appear uniform and healthy without signs of stress or contamination.
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