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Human Dermal Fibroblasts, Adult

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Cells are isolated from adult abdominal dermal explants and provided either cryopreserved or in a variety of plated formats. Dermal Fibroblasts are available from donors with and without Type 2 diabetes.
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Home | Primary Cells | Human Primary Cells | Others | Human Dermal Fibroblasts, Adult

Derived from adult skin’s dermal layer, Human Dermal Fibroblasts, or HDFa, are instrumental in synthesizing the skin’s connective tissue matrix. These fibroblasts are pivotal players in wound healing, where they utilize Fibronectin (FN) to facilitate their migration. Beyond wound repair, they engage in intricate interactions with epidermal cells during hair development and interfollicular skin processes. Noteworthy is their pivotal role in the groundbreaking generation of induced pluripotent stem cells (iPSC) alongside synovocytes. This achievement, made possible by the “Yamanaka cocktail” containing Oct3/4, Sox2, Klf4, and c-Myc, garnered Dr. Shinya Yamanaka the 2012 Nobel Prize in recognition of its transformative impact.

 

Why choose Human Dermal Fibroblasts, Adult from AcceGen?

Human Dermal Fibroblasts, Adult (HDFa), are cryopreserved at passage one and supplied frozen, with each vial containing over 5×105 cells in 1 ml. They exhibit spindle morphology and fibronectin-specific immunofluorescence. Tested negative for various pathogens, including HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi, they offer safety assurance. HDFa can reliably expand for up to 15 population doublings under AcceGen’s specified conditions.

 

Product Code

HDF-a
Species

Human
Cat.No

ABC-TC3029
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Others
Size/Quantity

1 vial
Cell Type

Fibroblast
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Dermal
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Dermal Cells

Citation

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AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • FOR RESEARCH USE ONLY

  • HDFa serve as a versatile model system for investigating various facets of cell physiology and have been prominently featured in numerous research works, especially within the realms of skin biology and reprogramming studies. These fibroblasts find application in diverse areas such as pathogen response, skin aging, wound healing, gene delivery, and skin-related disorders like scleroderma. Their utility extends to research involving ECM protein analysis, collagen metabolism, cosmetics, skin therapy, and model development, encompassing an array of multidisciplinary research avenues.

Frequently Asked Questions

  • What are Human Dermal Fibroblasts (HDFs)?

    Human Dermal Fibroblasts (HDFs) are cells derived from the dermis layer of human skin. They play a crucial role in the synthesis and remodeling of the extracellular matrix and are essential for wound healing and skin maintenance.

  • How do I ensure the optimal growth and health of HDFs in culture?

    To ensure optimal growth and health of HDFs, monitor cell confluency regularly and passage cells when they reach 80-90% confluency. Avoid over-confluency to prevent cell differentiation and senescence. Handle cells gently to avoid mechanical stress and maintain consistent culture conditions.

  • What are the morphological characteristics of HDFs in culture?

    In culture, HDFs typically exhibit a spindle-shaped, elongated morphology with prominent nuclei. They tend to grow in a monolayer with characteristic fibroblastic extensions. The cells should appear uniform and healthy without signs of stress or contamination.

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  • Fibroblast (273)
  • Human (1729)
  • Normal (1578)
  • Skin (157)

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