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Human Brain Microvascular Endothelial Cells

  • BSL

    1

  • 102
Human Brain Microvascular Endothelial Cells were initiated by elutriation of dispase dissociated normal human brain cortex tissue. These cells were originated using Complete Serum-Free Medium Kit, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells.
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Home | Primary Cells | Human Primary Cells | Nervous System Primary Cells | Human Brain Microvascular Endothelial Cells
Species

Human
Cat.No

ABC-TC3524
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Endothelial
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Brain
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Nervous Cells
Key Features

-Backed by AcceGen advanced technology
-Ship cryopreserved cells in dry ice
-Quality-tested for accurate results

Description

Human Brain Microvascular Endothelial Cells (HBMECs), derived from human brain, are one type of brain endothelial cells and the central element of the microvasculature which forms the blood-brain barrier (BBB). HBMECs limit the passage of soluble and cellular substances from the blood into the brain and shield the brain against toxins and immune cells. Brain microvascular endothelial cells can express cell adhesion molecules on their surface to regulate the extravasation of leukocytes into the brain. However, extracellular host-pathogen interactions can trigger calcium signaling in the endothelial cells, causing pathogen penetration through the BBB.
Why Choose Human Brain Microvascular Endothelial Cells from AcceGen?
AcceGen cryopreserves our Human Brain Microvascular Endothelial Cells for over 5 x 10^5 cells/mL within passage 3, for the best viability and plating efficiency after extensive testing and optimizations. Human Brain Microvascular Endothelial Cells express vWF/Factor VIII, CD31 (PECAM), or VE-Cadherin.

GFP-Human Brain Microvascular Endothelial Cells (GFP-HBMECs) and Immortalized Human Brain Microvascular Endothelial Cells (IM-HBMECs) are also available in AcceGen.

AcceGen Frozen Cells & Cell Lines 1 vial
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Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

  • For research use only.

  • Study the molecular and cellular properties of blood-brain barrier.
  • Brain endothelial cells in vitro can be used as the model of the blood-brain barrier (BBB) to study the nervous system diseases.

Frequently Asked Questions

  • What are the primary research applications of Human Brain Microvascular Endothelial Cells?

    These cells are commonly used in studies of the blood-brain barrier, neuroinflammation, and drug transport across the brain endothelium.

  • What are the recommended culture conditions for Human Brain Microvascular Endothelial Cells?

    These cells should be cultured in a specialized endothelial growth medium, under standard cell culture conditions (37°C, 5% CO2).

  • What functional assays can be used with Human Brain Microvascular Endothelial Cells?

    Functional assays for Human Brain Microvascular Endothelial Cells include permeability assays, transendothelial electrical resistance (TEER) measurements, and migration assays. These assays are used to study the barrier properties and dynamics of these cells.

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