Human Adipose Microvascular Endothelial Cells

Description

Human Adipose Microvascular Endothelial Cells (HAMVECs) are primary endothelial cells isolated from subcutaneous adipose tissue of healthy human donors. These cells exhibit a cobblestone-like morphology under standard culture conditions. Functionally, HAMVECs contribute to adipose tissue homeostasis by forming selective vascular barriers, facilitating fatty acid transcytosis, and secreting PPARγ ligands that modulate adipose tissue metabolism. They are implicated in obesity-related pathologies, including insulin resistance and chronic inflammation, through palmitate-induced activation of TLR4/NF-κB signaling pathway, which impair insulin delivery and promote monocyte adhesion. These cells express high levels of endothelial markers such as CD31, CD34, and von Willebrand factor (vWF), and exhibit depot-specific transcriptional profiles enriched in lipid-handling and Notch signaling pathways. As primary cells, HAMVECs have limited proliferative capacity, requiring low passage numbers for optimal viability and functionality.

Application

• FOR RESEARCH USE ONLY

• Human Adipose Microvascular Endothelial Cells (HAMECs) find significant applications in various fields of research and medicine. Due to their role in angiogenesis, coagulation, and inflammatory responses, these cells are invaluable for studying vascular-related diseases, such as cardiovascular disorders and inflammation-associated conditions. Their secretion of PPARγ ligands makes them a valuable tool for investigating adipose tissue metabolism and lipid regulation, with potential applications in understanding obesity and metabolic disorders. Furthermore, HAMECs’ capacity to promote preadipocyte proliferation positions them as critical players in studies related to adipogenesis and tissue development.