HighQC™ SACK-Xn 3C5 Adult Mouse Hair Follicle Stem Cells

Growing and expanding primary, differentiated adult cells in culture is difficult and laborious because many differentiated cells can only be maintained in vitro for a limited period of time. However, for controlled experiments, it may be necessary to maintain a consistent population of cells.

Maintaining a population of adult stem cells (ASCs), allows one to exponentially propagate a population of cells in vitro, and induce differentiation when desired. This is possible with SACK cell strains and specified cell culture conditions.

SACK ASC cell properties – Long-term propagation in xanthine (Xn)-supplemented medium

Suppressed asymmetric cell kineticsExpress the epithelial stem cell biomarker LGR-5Xn-suppressed non-random sister chromatid segregation
Differentiated progeny cell properties – Xn-free medium, confluent monolayer, serum reduction

Sebocyte differentiation as well as other undefined cell typesInduced expression of K5, K10, and filaggrin