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Species | Mouse |
Cat.No | ABC-SC0103T |
Product Category | Stem Cells |
Size/Quantity | 1 vial |
Cell Type | Fibroblast |
Shipping Info | Dry Ice |
Growth Conditions | 37 ℃, 5% CO2 |
Source Organ | Embryo |
Storage | Liquid Nitrogen |
Product Type | Animal Embryonic Stem Cells |
Host Cell | Mouse Embryonic Fibroblasts |
Mouse Embryonic Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Embryonic Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2×10^6 cells per ml and is delivered frozen. Mouse Embryonic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1: 2 or 1: 3 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.
When you publish your research, please cite our product as "AcceGen Biotech Cat.# XXX-0000". In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
For research use only