HighQC™ Mouse IPS Cell Line

Mouse induced pluripotent stem (iPS) cells were generated from mouse embryonic fibroblasts (MEFs) through the introduction of Oct3/4, Sox2, Klf4, and c-Myc genes using retroviral expression. Selection of the cells was based on their morphological characteristics, without the need for fluorescent markers or drug selection. These mouse iPS cells exhibit an indistinguishable morphology from mouse embryonic stem (ES) cells when cultured under standard ES cell conditions. They express key pluripotency markers such as SSEA-1 and Nanog, along with robust endogenous alkaline phosphatase activity. To support their growth, mouse iPS cells are cultured on a feeder layer of MEFs, necessitating pretreatment of the culture plate with Gelatin.

Why choose HighQC™ Mouse iPS Cell Line from AcceGen?

The Mouse iPS cell line from AcceGen is an excellent choice for several reasons. Firstly, we offer low passage cells with long-term viability, ensuring the maintenance of cell quality and functionality over extended periods. Secondly, these cells are readily available off the shelf, allowing for simple thawing and plating onto a serum-free, feeder-free culture system. This convenience saves time and effort in the laboratory. Lastly, the Mouse iPS cell line from AcceGen guarantees homogeneity as it originates from a single iPSC clone. This ensures consistency and uniformity in experimental results, making it an ideal choice for research and scientific studies.