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HighQC™ C57BL/6 Mouse Embryonic Fibroblasts | ||||
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| Product Name | HighQC™ C57BL/6 Mouse Embryonic Fibroblasts | |||
| Price | Get Quote | |||
| Cat.No | ABC-SC0101T | Species | Mouse | |
| Size/Quantity | 1 vial | |||
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description | C57BL/6 Mouse Embryonic Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Embryonic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 2×10^6 cells per ml and is delivered frozen. Mouse Embryonic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. Cells can be expanded for 3-6 passages at a split ratio of 1: 2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. | |||
| Disease | Normal | |||
| Source Organ | Embryo | |||
| Recommended Medium And Supplement | ABM-SM0101T HighQC™ C57BL/6 Mouse Embryonic Fibroblasts Medium Kit | |||
| Citation Guide | When you publish your research, please cite our product as "AcceGen Biotech Cat.# XXX-0000". In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | For research use only | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | Fibroblast | |||
| Growth Mode | Adherent | |||
| Product Type | Animal Embryonic Stem Cells | |||
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