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| Product Code | HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 |
| Species | Human |
| Cat.No | ABC-TC0381 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Liver Cell Lines |
Hep-G2, a cell line with an epithelial-like morphology, originates from a fascinating medical history. Initially isolated from a 15-year-old male youth diagnosed with liver cancer, it was initially misclassified as hepatocellular carcinoma before being correctly identified as hepatoblastoma. These cells, characterized by an average diameter of 10-20 micrometers, large nuclei with 3-7 nucleoli, and low mitochondrial content, exhibit specific chromosomal abnormalities associated with hepatoblastomas. Notably, they carry a TERT promoter mutation (C228T) and maintain a wild-type TP53, which is crucial in cancer development. In terms of cytochrome P450 (CYP) enzyme expression, HepG2 cells differ from normal hepatocytes, displaying weak or absent expression of several CYP enzymes involved in xenobiotic metabolism in the liver.
Why choose Hep-G2 from AcceGen?
Hep-G2 cells from AcceGen exhibit exceptional features, including high viability and quality. These cells are maintained in a sterile environment with daily monitoring to guarantee the absence of bacteria and fungi. Furthermore, they are confirmed to be free of mycoplasma contamination, and their identity is verified through STR analysis, ensuring rigorous quality control.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Ding, L. and Liang, X., 2021. Ras related GTP binding D promotes aerobic glycolysis of hepatocellular carcinoma. Annals of Hepatology, 23, p.100307.
FOR RESEARCH USE ONLY
The Hep-G2 cell line, derived from hepatoblastoma, holds a prominent position in biological science research, particularly in the context of xenobiotic metabolism and elimination studies. Although possessing certain limitations, Hep-G2 cells have proven invaluable in drug metabolism investigations and toxicity assessments. Notably, these cells exhibit a congruence in the expression of drug metabolism and transport proteins observed in both hepatocellular carcinoma and hepatoblastoma cells, making them a relevant model. Ongoing efforts are diligently focused on amplifying the cytochrome expression within Hep-G2 cells to enhance their utility as a hepatocyte model. Furthermore, researchers are actively exploring three-dimensional spheroid cultures to establish a more physiologically relevant platform, as these models demonstrate heightened metabolic activity compared to conventional two-dimensional cell cultures.
